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生物合成基因集群的鉴定和分析,用于含有化物的阿里尔聚烯西巴胺

Seiji Kawai¹, Akito Yamada¹, Danyao Du¹

¹Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

ACS chemical biology

|July 27, 2023

概括

研究人员阐明了spinamycin的生物合成,这是一个具有-键的天然产品. 他们发现酸对于形成化组至关重要,扩大了对微生物二次新陈代谢的知识.

科学领域:

微生物天然产品生物合成
有机化学有机化学
生物化学生物化学

背景情况:

具有- (N-N) 键的天然产品具有显著的生物活性.
越来越多地认识到依赖酸的N-N键形成途径.
使用酸合成化的精确催化机制在很大程度上仍未被阐明.

研究的目的:

为了研究spinamycin的生物合成途径,一个含有化的aryl polyene来自Streptomyces albospinus.
阐明酸在螺旋氨酸内化物部分的形成中的作用.
为了确定参与螺旋氨酸生物合成的关键酶和中间体.

主要方法:

基因组分析以识别spinamycin生物合成基因集群 (*spi*).
基因失活实验以证实集群在螺旋胺素生产中的作用.
稳定同位素标记 (酸) 的养实验.
采用纯化酶 (SpiA3,SpiA7) 的体外酶分析.

主要成果:

编码II型多基酸合成酶和酸生物合成酶的*spi*基因集群被确定并证实对螺旋氨酸生产至关重要.
稳定同位素标记表明,酸为化组提供一个原子.
实验室研究显示,N-N键形成发生在启动后的基质加载,并确定SpiA7作为一种催化二化的关键酶.

结论:

这项研究提出了一个全面的生物合成途径,用于spinamycin.
酸通过SpiA7.7催化的二化机制在化基组的形成中发挥着关键作用.
这项研究显著提高了对微生物二次新陈代谢中N-N键形成策略的理解.

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