香MKK1通过MK1-MPK6-3/11-4-bZIP21模块调节水果的成熟
Chaojie Wu1, Wei Deng2, Wei Shan1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, China.
Cell reports
|July 27, 2023
概括
控制香果成熟的新型监管途径涉及MaMKK1-MaMPK模块. 这一途径,包括MaMPK6-3/11-4和MabZIP21,通过酸化和转录激活影响水果成熟.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 线素激活蛋白激酶 (MAPK) 级联对植物发育至关重要.
- 在香果成熟调节中MKK的作用在很大程度上是未知的.
- 之前的研究表明,在香成熟过程中,MaMPK6-3会对MabZIP21产生酸化.
研究的目的:
- 阐明香果成熟过程中MKK的调控机制.
- 描述MaMKK1在香果成熟中的作用.
- 为了确定控制水果成熟的MAPK级联中的分子相互作用.
主要方法:
- 对MaMKK1.1的基因特征和表达分析.
- 香果中的MaMKK1暂时过度表达和沉默.
- 在体外激酶测试以确定酸化部位和活性.
- 分析蛋白质与蛋白质相互作用和转录调节.
主要成果:
- 在香果成熟过程中诱导MaMKK1的表达.
- 过度表达MaMKK1会加速成熟,而沉默会抑制成熟.
- MaMKK1可化并激活MaMPK6-3和MaMPK11-4.
- MaMPK11-4可化MabZIP21,增强其转录活性.
- 转基因番茄中的MabZIP21表达加速成熟.
- 在MabZIP21激活MaMPK11-4和MaMKK1转录的地方存在一个积极的反循环.
结论:
- 一个涉及MaMKK1,MaMPK6-3/11-4和MabZIP21的调节途径控制了香果的成熟.
- 该模块通过MPK激活和MabZIP21介导的转录变化来调节成熟.
- 这些发现揭示了一种复杂的反机制,控制着水果的成熟.
关键词:
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