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斯氏菌 suis 血清型 4 克隆复合体 94 的基因组特征和毒性从人类和猪样本中恢复
Rujirat Hatrongjit1, Parichart Boueroy2, Piroon Jenjaroenpun3
1Faculty of Science and Engineering, Department of General Sciences, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
PloS one
|July 27, 2023
概括
葡萄球菌 suis 血清型 4 菌株,特别是克隆复合体 94,在人类和猪中表现出潜在的毒性和致病性. 这些动物性传染性细菌具有毒性基因,并导致细胞死亡,因此需要监测.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 瑞杆菌 (Streptococcus suis) 是一种重要的动物性病原体,在人类和猪中引起侵入性感染.
- 血清型4菌株,特别是在克隆复合体 (CC) 94内,得到越来越多的认可,但它们的致病潜力需要进一步阐明.
研究的目的:
- 为了研究Streptococcus suis CC94血清型4菌株的基因组特征和体外毒性.
- 将这些菌株与已知的毒性S. suis血清型进行比较,并评估它们对人类细胞系的细胞毒性影响.
主要方法:
- 全基因组测序和对7个来自人类和猪来源的S. suis CC94血清型4菌株的比较基因组分析.
- 在体外细胞毒性测定使用人类肺腺癌和HeLa细胞.
- 检测抗微生物药物耐药性的基因.
主要成果:
- 所有七种S. suis CC94血清型4菌株都聚集在最小核心基因组组3 (MCG-3) 中,并携带了许多与毒性相关的基因.
- 细胞毒性测试表明,在与这些菌株进行化后,细胞活力的快速丧失,与毒性2型血清控制菌株相比.
- 确定了常见的抗菌素耐药性基因 (tet(O, ermB),使其对四环素,宏类和林科萨米德产生耐药性.
结论:
- 瑞波真菌CC94血清型4菌株表现出潜在的致病性和毒性特征.
- 这些发现凸显了对这些动物性传染性细菌的持续监测和监测的需要.
- 与毒性血清型2菌株的基因组相似性强调了S. suis血清型4菌株对公共健康的潜在风险.
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