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在类风湿性关节炎患者中对外体miRNAs的脱调
Muhammad Zahid Hussain1, Muhammad Shahbaz Haris2, Muhammad Rizwan2
1Department of Rheumatology, National University of Medical Sciences, Rawalpindi, Pakistan.
PloS one
|July 27, 2023
概括
外体微RNAs (miRNAs) 在类风湿性关节炎 (RA) 患者中显著下调,与增加的氧化应激和基因素脱甲基化相关. 这些放松调节的miRNAs显示出 RA 病变发生的生物标志物的潜力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 外基因组通过它们的载荷调解细胞间的通信,包括微RNA (miRNA).
- 失调的miRNAs通过改变蛋白质输出来促进疾病的发病.
- 在类风湿性关节炎 (RA) 发病过程中对外体miRNAs的研究有限.
研究的目的:
- 研究特定的外体miRNAs (miRNA-103a-3p,miRNA-10a-5p,miRNA-204-3p,miRNA-330-3p和miRNA-19b) 在RA病变发生中的作用.
- 探索这些外体miRNAs与RA患者的氧化应激和基因素脱乙烯化相关性.
主要方法:
- 定量实时PCR (qRT-PCR) 用于评估306名RA患者和对照者的外体miRNA表达水平.
- 与酶相关的免疫吸收试验 (ELISA) 用于测量氧化应激和基因素脱乙烯化水平的8-氧化 (8-OHdG).
- 包括相关性和接收器操作特征 (ROC) 曲线分析在内的统计分析.
主要成果:
- 与对照组相比,在RA患者中,所有五种研究的外体miRNA的表达显著下调 (p<0.0001).
- 在RA患者中显著增加氧化应激水平 (8-OHdG) 和基因素脱甲基化 (p<0.0001).
- 在放松调节的外体miRNAs和增加的氧化应激/激素脱乙烯化之间观察到负相关性.
- 在RA中,ROC分析表明这些miRNA的良好诊断潜力.
结论:
- 这项研究确定了RA患者特定外体miRNAs的显著下调.
- 脱调节的外体miRNAs与RA中氧化应激增加和基因素脱甲基化有关.
- 这些外体miRNAs可能在RA发作和进展中发挥作用,并可能作为诊断生物标志物.
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