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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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相关实验视频

Updated: Jul 21, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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基于Origami的单链RNA表观遗传免疫调节

Kun Dai1, Chen Gong2, Yang Xu3

  • 1School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Nano letters
|July 27, 2023
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种转录方法,精确地将功能分子添加到RNA纳米结构中. 这创造了先进的RNA原形,为疾病治疗提供了新的生物医学能力.

关键词:
增强型干扰素化疗是强化干扰素的化疗.表观遗传修饰是一种表观遗传修饰.核酸纳米技术 核酸纳米技术单链RNA原始创作

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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

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Nanomanipulation of Single RNA Molecules by Optical Tweezers

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相关实验视频

Last Updated: Jul 21, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
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科学领域:

  • 生物化学 生物化学
  • 纳米技术纳米技术
  • 分子生物学分子生物学

背景情况:

  • RNA纳米结构为分子应用提供了多功能平台.
  • 核类型的量子整合到RNA纳米结构是具有挑战性和未被充分探索的.
  • 了解核糖类相应物对RNA纳米结构功能的影响至关重要.

研究的目的:

  • 开发一种基于转录的方法,用于控制核糖类相似物与RNA纳米结构的集成.
  • 调查集成核酸相似物对RNA纳米结构的结构,稳定性和功能的影响.
  • 探索功能化RNA纳米结构在生物医学应用中的潜力.

主要方法:

  • 一种基于转录的方法被用来合成2000核酸单链RNA (ssRNA) 原始纳米结构.
  • 在分子层面上,多个核类相应物被可控地集成到ssRNA原形中.
  • 分析了集成ssRNA原始体的形态学,生物稳定性和生物医学功能.

主要成果:

  • 核类同类的整合并没有损害ssRNA原始的形态或生物稳定性.
  • 表观核酸相似物赋予了先天的免疫识别和调节功能.
  • 治疗性核糖类类似物增强了对瘤细胞杀伤的协同效应.
  • 开发的方法允许对功能性核糖类相似物进行定量整合.

结论:

  • 一种基于转录的新方法使得功能性核糖类相似物在分子水平上精确地集成到RNA纳米结构中.
  • 功能化的RNA纳米结构表现出保存的结构完整性和增强的生物医学功能.
  • 这种方法为开发多功能RNA原形为各种应用,特别是生物医学铺平了道路.