从食品废弃物中深入了解导向酸盐生产:微生物学解释和设计实践
Menghan Wu1, Xinning Liu1, Weiming Tu2
1State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
Water research
|July 27, 2023
概括
这项研究提出了一种新的"食品废物-乳酸盐-酸"发酵路线,使用混合微生物培养 (MMC) 来有效地生产酸,一种有价值的化学物质,克服在开放环境和复杂废物流中的挑战.
科学领域:
- 生物技术是生物技术.
- 微生物生态学 微生物生态学
- 生物化学工程 生物化学工程
背景情况:
- 使用混合微生物培养 (MMCs) 从复杂废物中产生酸等特定挥发性脂肪酸 (VFA) 是具有挑战性的,因为微生物的多样性和过程的复杂性.
- 富含碳水化合物的废物通常有利于酸盐的生产,而不是在单阶段无氧过程中具有价值的奇偶碳VFAs,如酸.
研究的目的:
- 研究使用MMC从乳酸中生产定向酸盐的可行性.
- 开发和验证一个两阶段的方法.
主要方法:
- 微生物信息学和反应分析,以评估从乳酸盐的酸产量.
- 一个两阶段的发酵过程:第一阶段使用定义的微生物组在pH 4下生产乳酸盐,第二阶段使用未定义的微生物组在优化的C/N比率 (100:1-200:1) 和pH 5.0下生产酸盐.
主要成果:
- 成功逆转酸盐主导发酵,在总VFA中达到45.26%±2.23%的酸.
- 证明在第一阶段的低pH值通过抑制非功能性细菌来稳定微生物群体.
- 确定虽然选择酸盐生产微生物至关重要,但对于稳定的酸盐类型发酵是不够的.
结论:
- 拟议的"食品废物-乳酸盐-酸"路径是从复杂废物流中导向酸生产的可行策略.
- 工程微生物群落和控制环境条件是实现稳定和高效VFA生产的关键.
- 本研究提供了一个框架,用于设计增强的MMC发酵工艺,以有针对性的VFA恢复.
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