在小麦中与定位时间调节相关的蒂奥雷素基因TaTrxh9的表征
Yadong Fan1, Mengyuan Li2, Yujie Wu1
1Henan Technology Innovation Center of Wheat, Henan Agricultural University, Zhengzhou, 450046, China; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China; State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450046, China.
Plant physiology and biochemistry : PPB
|July 27, 2023
概括
研究人员鉴定并描述了小麦氧素基因TaTrxh9.9的特征. 这种基因在调节小麦出货时间方面发挥着至关重要的作用,为早期出货品种的分子育种提供了新的途径.
科学领域:
- 植物分子生物学 植物分子生物学
- 转毒生物学 转毒生物学
背景情况:
- 铁素 (Trxs) 是植物中重要的氧化还原调节蛋白质,但它们在小麦中的功能在很大程度上仍未被探索.
- 小麦是全球重要的粮食作物,因此对其遗传调节的理解至关重要.
研究的目的:
- 在小麦中识别和功能性地表征h型 thioredoxin 基因 (TaTrxh9).
- 调查TaTrxh9在小麦发育中的作用,特别是在标题时间调节方面.
主要方法:
- 在小麦中鉴定和测序分析TaTrxh9同质物.
- 在体外酶活性测定和TaTrxh9蛋白的结构建模.
- 在小麦的Arabidopsis和基因沉默/淘汰研究中的功能补充.
主要成果:
- TaTrxh9具有保存的硫素域和活性位点,在体外表现出催化活性.
- 过度表达TaTrxh9拯救了Arabidopsis突变表型,证实了它的功能.
- TaTrxh9在小麦组织中具有差异性表达,其抑制/淘汰会导致加快定位.
结论:
- TaTrxh9是一种功能性硫素,参与小麦发育.
- 这项研究确定了硫素h型和小麦的标题时间调节之间的新联系.
- TaTrxh9为分子育种提供了潜在的目标,以开发具有早期标题特征的小麦品种.
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