相关实验视频

Updated: Jul 21, 2025

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

9.9K

INTERPIN:一种用于细菌内在转录终止毛针的存储库

Swati Gupta1, Namrata Padmashali1, Debnath Pal1

  • 1Department of Computational and Data Sciences, Indian Institute of Science, Bengaluru, 560012, Karnataka, India.

Biochimie
|July 27, 2023
PubMed
概括

研究人员发现了新的集群针头,可以促进细菌的内在转录终止 (ITT). 这一发现扩大了对基因调节的理解,并为分析细菌基因组提供了一个新的工具 - - INTERPIN.

关键词:
细菌基因组中的细菌基因组.数据库数据库数据库是一个数据库.头发针头的头发针头是什么意思转录终结是独立于罗的转录终结.

更多相关视频

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly09:51

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

7.7K
In Vitro Transcription Assays and Their Application in Drug Discovery09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

15.2K

相关概念视频

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.5K
Transcription in Prokaryotes01:28

Transcription in Prokaryotes

Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow...
93
Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.5K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.7K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.3K
Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.5K