盐可诱导酶通过增强CREB信号传递来调节theca细胞中的雄激素合成
Miriam Rodriguez Esquivel1, Emily Hayes1, Oliwia Lakomy1
1Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL, 60612, USA.
Molecular and cellular endocrinology
|July 27, 2023
概括
盐诱导激酶 (SIKs) 调节卵巢theca细胞 (TCs) 中的雄激素产生. 抑制SIKs可以增强黄素化激素的作用.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞信号传输 细胞信号传输
背景情况:
- 太卡细胞 (TCs) 对于卵巢雄激素合成至关重要,对女性生育至关重要.
- 在TC中失调的雄激素生产与不孕症和高雄激素状况有关.
- 已知盐诱导性激酶 (SIKs) 能够调节颗粒细胞中的类固醇生成.
研究的目的:
- 调查SIKs在调节卵巢TCs内的雄激素生产中的作用.
- 确定SIK是否代表TC功能中的新型监管机制.
主要方法:
- 在小鼠TC中检测SIK2和SIK3表达 (体内和体外).
- 用素化激素 (LH) 和SIK抑制剂治疗培养的TC.
- 评估类固醇基因表达和雄激素生产.
- 涉及福斯科林和循环AMP (cAMP) 的实验,以探测信号通路.
- 在SIK2淘汰赛小鼠中分析循环水平.
主要成果:
- 在TC中存在SIK2和SIK3.
- 抑制SIK增强了LH刺激的雄激素生产和类固醇基因表达.
- 仅仅SIK抑制就增加了雄激素的产生和基因表达.
- 显然,SIKs在cAMP信号的下游调节目标.
- 在SIK2淘汰赛中,小鼠的血液中丸激素水平升高.
结论:
- SIKs以TC表达,并在调节雄激素合成中发挥重要作用.
- 抑制SIK可以增强TC功能和雄激素的产生,可能通过cAMP通路.
- SIKs代表了一种新的治疗点,用于管理高雄性障碍.
相关概念视频
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
MAPK Signaling Cascades
5.7K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.7K
cAMP-dependent Protein Kinase Pathways
6.4K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.4K
PI3K/mTOR/AKT Signaling Pathway
3.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.7K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K


