该c-di-GMP信号组件YfiR调节多种细菌表型和Pseudomonas plecoglossicida中的毒性
Ying Wang1, Yinhua Jin1, Fei Sun1
1State Key Laboratory of Bioreactor Engineering, Laboratory for Aquatic Animal Diseases, East China University of Science and Technology, Shanghai 200237, China.
Journal of applied microbiology
|July 27, 2023
概括
在Pseudomonas plecoglossicida中,YfiR调节周期性迪加尼酸盐 (c-di-GMP) 水平,影响生物膜形成和毒性. 在斑马鱼模型中删除YfiR减弱的细菌殖民和疾病.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 水生动物卫生 水生动物卫生
背景情况:
- Pseudomonas plecoglossicida 致使大黄的内脏颗粒瘤疾病,导致严重的经济损失.
- 生物膜的形成对P. plecoglossicida的生活方式至关重要,由细胞内循环bis (3'-5') diguanylic acid (c-di-GMP) 水平调节.
研究的目的:
- 为了研究YfiR的作用,一个调节器的 diguanylate环酶YfiN,在控制c-di-GMP水平.
- 阐明YfiR在P. plecoglossicida中调节细菌毒性表达中的功能.
主要方法:
- 基因分析以确定参与c-di-GMP监管的yfiBNR操作子.
- 一个yfiR突变的构建和特征.
- 建立斑马鱼幼虫黄囊微注射感染模型.
主要成果:
- yfiR突变体表现出增加的c-di-GMP水平,增强的生物膜形成,以及更大的外聚糖类生产.
- 这种yfiR突变体的游泳和群体活动能力下降.
- 在斑马鱼模型中,yfiR突变体表现出衰弱的毒性,恢复了宿主生存率和减少了细菌殖民.
结论:
- 鉴定出YfiR是P. plecoglossicida中毒性的关键调节者.
- YfiR影响c-di-GMP水平,生物膜的形成,外聚糖的产生,运动性和细菌的殖民.
- 针对YfiR可能是控制水产养殖中P. plecoglossicida感染的战略.
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