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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

64
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass
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扩展的CRISPR工具箱用于构建微生物细胞工厂.

Yuxi Teng1, Tian Jiang1, Yajun Yan1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, GA 30602, USA.

Trends in biotechnology
|July 27, 2023
PubMed
概括

克里斯普技术的进步显著提高了微生物细胞工厂 (MCF) 的建设. 扩展的CRISPR工具箱使精确的基因工程能够从简单的碳来源产生有价值的化合物.

科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 微生物细胞工厂 (MCF) 对于将低成本的碳来源转化为有价值的化合物至关重要.
  • 由于CRISPR/Cas9系统的可编程性,它已经将基因组编辑转化为MCF结构.

研究的目的:

  • 总结最近在CRISPR工具箱开发中的进展,用于MCF构建.
  • 突出扩展CRISPR系统在基因编辑,转录调节和酶调节中的应用.

主要方法:

  • 对新型CRISPR系统和工程Cas效应器的审查.
  • 探索用于多层调节和双链无断编辑的CRISPR应用.
  • 分析CRISPR在途径构建,酶工程和代谢控制中的作用.

主要成果:

  • 扩展的CRISPR工具箱为MCF工程提供了增强的可编程性和精度.
  • 新的CRISPR系统促进了多级调节,并使基因编辑能够在没有双链断裂的情况下进行.
  • 克里斯普尔工具有效地支持MCF中的途径构建,酶优化和代谢负担管理.

结论:

  • 不断发展的CRISPR工具箱是推动微生物细胞工厂设计和应用的强大资产.
关键词:
这是一个CRISPR工具箱.基础编辑 基础编辑代谢工程是代谢工程.微生物细胞工厂是一个微生物细胞工厂.合成的代谢 它们可调节的规则 调节规则

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  • 未来的CRISPR技术的开发和整合有望在合成生物学和生物制造领域进一步创新.