菌体对肉中沙门氏菌感染的作用
Zahra Sarrami1, Mohammad Sedghi2, Ishmael Mohammadi1
1Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Scientific reports
|July 27, 2023
概括
菌体 (BP) 在 brojlers 中显著减少了沙门氏菌和大肠杆菌. 从第一天开始用BP补充肉饮食,可以有效防止沙门氏菌的传播,并改善肠道健康.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 微生物学 微生物学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 沙门氏菌 (SE) 感染对肉的健康和生产力构成重大威胁.
- 抗生素耐药性需要替代策略来控制家禽中的细菌感染.
- 菌体 (BP) 为控制肉中的沙门氏菌等细菌病原体提供了一个有希望的替代方案.
研究的目的:
- 评估菌体 (BP) 在控制肉中沙门氏菌 (SE) 感染的疗效.
- 评估BP补充剂对肠道健康,细菌负载和肝脏病理的影响.
- 为了比较BP与胆固醇的作用,一种常用的抗生素,在管理SE感染.
主要方法:
- 一项完全随机的设计研究,涉及五个治疗组的150只一天大的肉.
- 治疗包括基础饮食,胆固醇补充剂和两种水平的菌体 (BP) 补充剂 (1g/kg和1.5g/kg).
- 在第13天,肉受到沙门氏菌 (SE) 的挑战,监测了受挑战和暴露 (非受挑战但共住) 群体.
主要成果:
- 与对照组相比,BP和胆固醇补充剂显著降低了眼膜和肝脏中SE和胆固醇菌的数量.
- 在暴露和受挑战的群体中,食BP或素的肉表现出改善的毛高度和表面积.
- 在受挑战和暴露的中,BP补充导致PPARγ基因表达减少和TLR4基因表达降低,表明炎症减少.
结论:
- 从第一天开始服用菌体 (BP) 的食补充剂,可以有效地防止沙门氏菌在肉中传播.
- 血压证明对肠道形态产生积极影响,并减少细菌负载,为抗生素提供了可行的替代品.
- 高血压治疗调节了关键基因表达 (PPARγ和TLR4),表明在沙门氏菌感染的背景下具有抗炎作用.
相关概念视频
01:30Lytic Cycle of Bacteriophages
70.9K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.9K
00:43Lysogenic Cycle of Bacteriophages
62.4K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.4K
01:26DNA Bacteriophages
52
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
52
01:20Viral Replication: Lytic Cycle
73
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
73
01:16Viral Replication: Lysogenic Cycle
76
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
76