相关实验视频
Updated: Jul 21, 2025
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
34.7K
全面的,无标签的,单分子可视化DNA原木纳米设备跨生物样本使用原木FISH
Wendy Xueyi Wang1, Travis R Douglas1, Haiwang Zhang2,3,4
1Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Nature nanotechnology
|July 27, 2023
概括
我们开发了origamiFISH,这是一种新的方法来追踪细胞和组织中的DNA纳米结构 (DNs). 这种无标签的技术为了解DN生物分布和指导治疗开发提供了高灵敏度.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 分子生物学分子生物学
背景情况:
- DNA原始结构纳米结构 (DNs) 对生物应用具有前景.
- 了解DN细胞相互作用和生物分布至关重要,但具有挑战性.
- 目前的追踪方法缺乏灵敏度,可能会导致文物.
研究的目的:
- 开发一种敏感的,无标签的方法来检测和跟踪生物系统中的DNA纳米结构.
- 研究DN在细胞和组织中的时空分布模式.
- 为了实现对DN分布的准确映射,用于治疗开发.
主要方法:
- 开发了origamiFISH,一种通用,无标签的方法,用于单分子光检测DNA.
- 采用混合化连锁反应探测器,针对脚手架序列进行1000倍的信号放大.
- 优化与免疫光和组织清除技术的兼容性.
主要成果:
- 取得了皮科莫拉检测灵敏度,比标准方法低1万倍.
- 在吸收几分钟内确定了细胞类型和DN形状特定的分布模式.
- 在各种复杂的生物样本中可视化DN分布,包括有机体和组织部分.
结论:
- origamiFISH提供了前所未有的灵敏度和准确性,用于在现场跟踪DNs.
- 能够通过亚细胞和组织屏障对DN生物分布进行详细的映射.
- 促进了基于DN的治疗方法的合理设计和开发.
更多相关视频
08:51Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
Published on: June 23, 2016
10.8K
10:43Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
Published on: July 21, 2023
3.4K
相关概念视频
02:07FISH - Fluorescent In-situ Hybridization
20.8K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
20.8K
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K