在未培养的古生物之间预测的CRISPR介导的共生
Sarah P Esser1,2, Janina Rahlff2,3, Weishu Zhao4,5
1Environmental Metagenomics, Research Center One Health Ruhr of the University Alliance Ruhr, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Nature microbiology
|July 27, 2023
概括
在古生物中,CRISPR系统向共生性表交生物体DNA. 这种在未经培养的古生物中观察到的防御机制表明,在古物系中针对CRISPR-Cas的独立进化.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 考古物 (Archaea) 是一种古老的物种.
背景情况:
- 克里斯普尔-卡斯系统是 prokaryotic 对外来DNA的防御机制.
- 它们在古生物中的作用,特别是对抗共生伙伴的作用,不太了解.
研究的目的:
- 在未培养的古生物中研究CRISPR-Cas系统,目标是古生物的表象生物.
- 了解这种相互作用的进化起源和功能影响.
主要方法:
- 转基因组学是指转基因组学.
- 转录代码组学 (Metatranscriptomics) 是一种关于转录代码组学的方法.
- 单细胞基因组学 单细胞基因组学
- 分析数据的meta-omics数据分析.
- 代谢相互作用建模的代谢相互作用建模
主要成果:
- 在Candidatus Altiarchaeum物种中,CRISPR系统针对它们的副共生生物 (Ca. 这是一个古老的古迹 (Huberiarchaeum).
- 在现场检测到表达的CRISPR间隔器.
- 副交生体的功能范围从寄生到互惠,这取决于宿主基因型.
结论:
- 克里斯普尔-卡斯对共生考古基因组的准在各种考古谱系中独立演变.
- 这突显了CRISPR-Cas在调节宿主同生体相互作用中的新作用.
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