相关实验视频

Updated: Jul 21, 2025

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

Published on: May 4, 2020

和铁单独或组合对BV2细胞中亡的作用

Yuan-Yuan Fang^1,2, Cui-Liu Gan^1,2, Jian-Chao Peng^1,2

¹Department of Toxicology, School of Public Health, Guangxi Medical University, 22 Shuang-Yong Rd., Nanning, 530021, Guangxi, China.

Biological trace element research

|July 27, 2023

概括

接触和铁会诱导BV2微质细胞的亡. 联合暴露显示出比单独的更低的细胞毒性,表明这些金属之间具有对抗作用.

科学领域:

神经科学是一个神经科学.
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

微质在中枢神经系统内的神经炎症和免疫反应中起着至关重要的作用.
接触和铁等重金属会影响微质功能,并导致神经退行过程.

研究的目的:

为了研究和铁对BV2微质细胞的亡的单独和联合作用.
为了确定与单个暴露相比,和铁的联合暴露是否会增加它们的毒性.

主要方法:

用小鼠微质BV2细胞系进行实验.
评估细胞活力,反应性氧物种 (ROS) 生产,促炎性细胞因子释放 (IL-1β,TNF-α) 和细胞亡,使用包括PI排除,DCFH-DA染色,ELISA和Annexin V/7-AAD染色在内的各种测试.
通过免疫阻塞和色度测定分别分析了亡信号通路 (caspase9,P53,Bax,Bcl2) 和caspase3活性.

主要成果:

和铁的暴露,单独或联合,激活了BV2微质,增加了ROS的产生,并增加了促炎性细胞因子的表达.
亡速率增加,线粒体膜潜力下降,与亡相关的关键蛋白质 (caspase9,P53,Bax,Bcl2) 和caspase3活性被上调.
与铁的联合暴露显示出较低的细胞毒性比单独的暴露,这表明了对抗性相互作用.

结论:

和铁,单独或组合,都会在质细胞中诱导细胞亡.
铁在BV2微质中表现出对诱导的毒性具有保护性或对抗性作用.

关键词:

它们是对立的对手.细胞灭亡 (apoptosis) 是一种死亡的过程.组合曝光组合曝光组合曝光铁铁铁是什么意思铁铁铁是一种.神经毒性神经毒性这就是ROSOS ROS.

更多相关视频

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Published on: August 3, 2008

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

相关概念视频

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

Apoptosis

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...