通过CIPK23的酸化调节了Arabidopsis中的高亲和度Mn转运器NRAMP1
Thibault Kosuth1, Alexandra Leskova1, Reyes Ródenas2
1IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro, Montpellier, 34060, France.
FEBS letters
|July 28, 2023
概括
植物根细胞使用NRAMP1来控制 (Mn) 的摄取. 研究人员将CIPK23确定为一种与NRAMP1相互作用的激酶,调节其运输并帮助维持植物中的Mn平衡.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 生物化学 生物化学
背景情况:
- (Mn) 是植物必需的微量营养素,对各种生理过程至关重要.
- 过度的Mn吸收导致毒性,需要严格调节Mn稳态.
- 根Mn转运器NRAMP1在Mn吸收和内部贩运中发挥着关键作用,在血膜和内体细胞之间循环.
研究的目的:
- 为了确定负责酸化和调节植物运输体NRAMP1.1活动的特定激酶.
- 为了阐明NRAMP1调节的基础分子机制,以响应的可用性.
- 为了研究CIPK23在植物平衡中的作用.
主要方法:
- 分裂露西法酶 (LUC) 查以确定NRAMP1.1.的激酶相互作用体.
- 分离-mCitrine双分子光补充 (BiFC) 来确认蛋白质-蛋白质相互作用.
- 同免疫沉 (Co-IP) 试验验证了NRAMP1-激酶的相关性.
- 试管体内激酶试验以确定NRAMP1.1上的酸化位点.
主要成果:
- 分裂露西法酶查确定了CIPK23作为NRAMP1.1的绑定合作伙伴.
- 分离-mCitrine BiFC和Co-IP测定证实了NRAMP1和CIPK23.23之间的相互作用.
- 实验室酸化试验显示,CIPK23针对NRAMP1的特定残留物,包括对NRAMP1内细胞化很重要的血清20.
- 在具有突变 cipk23 的植物中,Mn诱导的NRAMP1内化没有显著影响,这表明与其他激酶的潜在功能冗余.
结论:
- CIPK23直接与植物载体NRAMP1相互作用并酸化,从而影响其内细胞形成.
- 虽然CIPK23被确定为一个关键调节器,但它的突变并没有取消Mn诱导的NRAMP1内化,这表明其他激酶可能会补偿其功能.
- 这项研究提供了对控制植物中恒温的复杂调节网络的洞察,突出了酶介导酸化在转运器贩运中的作用.
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