相关实验视频
Updated: Jul 21, 2025

11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
10.9K
LINC01088/miR-22/CDC6 轴通过激活PI3K/AKT通路来调节前列腺癌的进展
Jianwei Li1, Xinghua Huang1, Haodong Chen1
1Department of Urology, Longgang District People's Hospital of Shenzhen, Guangdong 518000, China.
Mediators of inflammation
|July 28, 2023
概括
这项研究揭示了LINC01088/microRNA-22/细胞分裂周期6轴对前列腺癌的影响. 这一轴涉及PI3K/AKT通路,为前列腺癌提供了潜在的诊断和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 前列腺癌 (PCa) 是对男性生殖系统的重大威胁.
- 长非编码RNAs (lncRNAs) 参与了PCa的发展.
- 研究了LINC01088/microRNA-22/细胞分裂周期6 (CDC6) 轴,以确定其在PCa中的作用.
研究的目的:
- 阐明LINC01088/miR-22/CDC6轴在前列腺癌进展中的作用.
- 研究涉及PI3K/AKT信号通路的调控机制.
- 确定PCa.潜在的诊断和治疗点.
主要方法:
- 使用基因表达综合和RT-qPCR对PCa组织和细胞的基因表达分析.
- 使用双露西法酶试验验的相互作用验证.
- 使用CCK-8和Transwell试验评估细胞活力,迁移和入侵.
- 分析PI3K/AKT通路通过西部斑块的激活.
主要成果:
- 在PCa中,LINC01088和CDC6是上调的,而miR-22是下调的.
- miR-22直接针对LINC01088和CDC6.6两个目标.
- 通过抑制PI3K/AKT通路,LINC01088的抑制抑制了PCa的进展.
- 过度表达CDC6抵消了LINC01088敲击的抑制作用.
结论:
- LINC01088/miR-22/CDC6轴是PCa进展的一个关键调节器.
- 这个轴通过PI3K/AKT信号通路影响PCa的发展.
- 已识别的轴为PCa诊断和治疗提供了一个有前途的目标.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
3.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.7K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The JAK-STAT Signaling Pathway
9.0K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.0K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Tumor Progression
6.4K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.4K

