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稳定酶不动化的水凝用于扩展的低毒细胞培养

Britney N Hudson¹, Camron S Dawes¹, Hung-Yi Liu²

¹Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202, USA.

Emergent materials

|July 28, 2023

概括

研究人员开发了葡萄糖氧化酶 (GOx) 固定化的水凝,以产生溶液缺氧. 优化的水凝在冷干燥后和细胞培养应用化期间保持酶活性.

科学领域:

生物材料科学生物材料科学
生物技术是生物技术.
生物化学生物化学

背景情况:

创建受控的低氧环境对于细胞培养研究至关重要.
诱导缺氧的现有方法可能很复杂或耗时.
葡萄糖氧化酶 (GOx) 是一种消耗氧气的酶,为低氧产生提供了潜在的解决方案.

研究的目的:

开发和优化葡萄糖氧化酶 (GOx) 固定化水凝,以产生溶液缺氧.
为了克服在水凝制造和使用过程中酶活性损失所带来的挑战.
为了在标准细胞培养化器中实现长期,方便的低氧诱导.

主要方法:

烯酸葡萄糖氧化酶 (GOx) 与聚乙烯糖醇 (PEGDA) 二烯酸盐 (PEGDA) 共聚合,形成PEGDA-GOx水凝.
研究了减轻冷干燥和过氧化 (反应副产品) 的负面影响的策略.
测试了诸如三/拉芬和谷氨 (GSH) 等添加剂,以保持GOx活性.

主要成果:

发现冷干燥和过氧化可以降低固定GOx.的消耗氧的活动.
添加三/拉芬保护GOx活动免受冷干燥损伤.
包括谷氨 (GSH) 阻止了过氧化对GOx的抑制.

结论:

优化的PEGDA-GOx水凝有效地保持葡萄糖氧化酶活性.
这些水凝为诱导细胞培养中的长期溶液缺氧提供了一种稳定且方便的方法.
开发的材料有助于细胞培养应用,需要在标准的二氧化碳化器中控制低氧条件.

关键词:

氧气过低是因为缺氧.癌症癌症癌症癌症癌症酶固定化酶固定化葡萄糖氧化酶是什么?这是一种水凝.

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