人类动脉动脉样硬化的高维单细胞多模式景观
Alexander C Bashore1, Hanying Yan2, Chenyi Xue1
1Division of Cardiology, Department of Medicine, Vagelos College of Physicians and Surgeons, Columbia University, New York.
medRxiv : the preprint server for health sciences
|July 28, 2023
概括
这项研究绘制了人类动脉动脉样硬化的细胞格局,揭示了25种不同的细胞类型及其在疾病中的作用. 通过详细介绍细胞群及其临床相关性,研究结果确定了动脉样硬化的新治疗点.
科学领域:
- 心血管生物学 心血管生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 动脉样硬化斑块是复杂的细胞结构,对转录和表型细胞格局的了解很少.
- 关于动脉样硬化病变中的多种细胞类型及其特定作用的知识有限.
研究的目的:
- 为了全面地描述人类动脉动脉样硬化的细胞和分子格局.
- 识别不同的细胞种群及其与临床病理生理学的关联.
主要方法:
- 结合单细胞RNA测序 (scRNA-seq) 和通过测序 (CITE-seq) 来对转录组和表位组进行细胞索引.
- 将所有细胞类型分为21种人类大脑动脉样硬化病变 (13种症状).
- 开发了一种流动细胞测量面板,用于分离流细胞衍生的细胞.
主要成果:
- 确定了25种独特的细胞群,具有独特的多原子特征,包括巨细胞,T细胞,B细胞和光滑肌细胞 (SMC).
- 发现了促炎性巨细胞子集,TREM2阳性泡细胞,增殖性子群和SMC衍生的泡细胞.
- 在症状患者的斑块中发现了减少的细胞巨细胞和丰富的炎症性SMC.
结论:
- 创建了动脉样硬化细胞种群的多式地图,提供了关于细胞多样性,表型,位置和临床相关性的见解.
- 促进对特定细胞类型的心血管疾病易感位点的映射.
- 识别了用于动脉样硬化治疗的新型分子和细胞治疗点.
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