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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.4K

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相关实验视频

Updated: Jul 21, 2025

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
04:52

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

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在倾斜的冷电磁数据收集过程中克服分辨率减弱.

Sriram Aiyer, Philip R Baldwin, Shi Min Tan

    bioRxiv : the preprint server for biology
    |July 28, 2023
    PubMed
    概括

    电子显微镜 (cryo-EM) 用户可以通过在数据收集过程中倾斜阶段来克服首选样本定向问题. 新的工作流程将分辨率损失降至最低,从而实现高分辨率的3D重建.

    科学领域:

    • 结构生物学 结构生物学
    • 生物物理学的生物物理.
    • 生物化学 生化学

    更多相关视频

    Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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    Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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    相关实验视频

    Last Updated: Jul 21, 2025

    Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
    04:52

    Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

    Published on: July 1, 2022

    2.4K
    Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
    08:16

    Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

    Published on: March 19, 2021

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    Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
    08:55

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    Published on: July 12, 2022

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    背景情况:

    • 在冷电子显微镜 (cryo-EM) 中首选的样本定向导致异构地图,并阻碍了准确的3D结构确定.
    • 标本阶段倾斜是一种已知的策略,可以减轻偏好的方向,但通常会导致显著的分辨率损失.

    结论:

    • 在冷EM数据收集过程中使用阶段倾斜是获得同otropic 图的高效策略.
    • 开发的方法和定量框架增强了阶段倾斜的实用性,以进行可靠的冷电磁结构确定.
    • 这种方法使得高分辨率的结构分析的标本,以前由首选的定向文物限制.