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Updated: Jul 21, 2025

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

在倾斜的冷电磁数据收集过程中克服分辨率减弱

Sriram Aiyer, Philip R Baldwin, Shi Min Tan

bioRxiv : the preprint server for biology

|July 28, 2023

概括

电子显微镜 (cryo-EM) 用户可以通过在数据收集过程中倾斜阶段来克服首选样本定向问题. 新的工作流程将分辨率损失降至最低,从而实现高分辨率的3D重建.

科学领域:

结构生物学结构生物学
生物物理学的生物物理.
生物化学生化学

背景情况:

在冷电子显微镜 (cryo-EM) 中首选的样本定向导致异构地图,并阻碍了准确的3D结构确定.
标本阶段倾斜是一种已知的策略,可以减轻偏好的方向,但通常会导致显著的分辨率损失.

结论:

在冷EM数据收集过程中使用阶段倾斜是获得同otropic 图的高效策略.
开发的方法和定量框架增强了阶段倾斜的实用性,以进行可靠的冷电磁结构确定.
这种方法使得高分辨率的结构分析的标本,以前由首选的定向文物限制.

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相关概念视频

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...