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Updated: Jul 21, 2025

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多维超分辨率显微镜揭示了FUS凝结物的老化中的纳米级表面聚合物

Changdong He, Chun Ying Wu, Wan Li

bioRxiv : the preprint server for biology

|July 28, 2023

概括

像FUS蛋白质这样的生物分子凝聚物经历衰老,在其表面形成固体粉样蛋白聚合物,阻碍内部扩散. 先进的超高分辨率显微镜揭示了这些纳米级变化在老化的FUS凝聚物.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

细胞内液体-液体相分离 (LLPS) 形成了对细胞功能至关重要的无膜有机体.
FUS蛋白质凝结物模拟LLPS衰老和与疾病相关的转变,但它们的内部结构和成熟机制尚不清楚.
传统的显微镜限制了微米大小的凝聚物的分辨率,阻碍了纳米尺度属性的研究.

结论:

揭示了单元FUS凝结物的纳米级结构重组和衰老机制.
展示了老化和机械应力如何促进表面粉样聚合物的扩张,改变凝结物的物理性质.
突出了协同超分辨率显微镜技术在剖析复杂的LLPS系统中的力量.

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