Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.5K
Inflammatory Response01:28

Inflammatory Response

2.1K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Meta-analysis Comparing Combined Use of Eicosapentaenoic Acid and Statin to Statin Alone.

The American journal of cardiology·2019
Same author

Correction to "MEMS impedance flow cytometry designs for effective manipulation of micro entities in health care applications" [Biosens. Bioelectron], 2019, 142, 111526.

Biosensors & bioelectronics·2019
Same author

Structure-function relationship of H2A-H2B specific plant histone chaperones.

Cell stress & chaperones·2019
Same author

Scope and Limitations of γ-Valerolactone (GVL) as a Green Solvent to be Used with Base for Fmoc Removal in Solid Phase Peptide Synthesis.

Molecules (Basel, Switzerland)·2019
Same author

Joint Consensus Statement of the Indian National Association for Study of the Liver and Indian Radiological and Imaging Association for the Diagnosis and Imaging of Hepatocellular Carcinoma Incorporating Liver Imaging Reporting and Data System.

Journal of clinical and experimental hepatology·2019
Same author

Do we have enough evidence to support the use of SGLT2 inhibitors for MACE reduction in patients with advance kidney disease?

European journal of internal medicine·2019

相关实验视频

Updated: Jul 21, 2025

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

7.1K

KSHV vIL-6通过表观遗传重编程增强炎症反应

Tomoki Inagaki1, Kang-Hsin Wang1, Ashish Kumar1

  • 1Department of Dermatology, School of Medicine, the University of California Davis (UC Davis), Sacramento, California USA.

bioRxiv : the preprint server for biology
|July 28, 2023
PubMed
概括

卡波西肉瘤相关的疹病毒 (KSHV) 炎症性细胞因子综合征 (KICS) 涉及高病毒载量和升高的IL-6. 长时间暴露于病毒IL-6 (vIL-6) 会产生表观遗传记忆,增加KSHV感染个体的炎症风险.

更多相关视频

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.5K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

相关实验视频

Last Updated: Jul 21, 2025

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

7.1K
Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.5K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

科学领域:

  • 免疫学 免疫学 免疫学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 病毒学 病毒学

背景情况:

  • 卡波西肉瘤相关疹病毒 (KSHV) 炎症性细胞因子综合征 (KICS) 是一种与KSHV感染相关的慢性炎症状况,其特征是高病毒载荷和KSHV编码的IL-6 (vIL-6) 和人类IL-6 (hIL-6) 的血清水平升高.
  • KICS与恶性瘤和其他并发症的风险增加有关,长时间暴露vIL-6被怀疑在疾病进展中起作用,尽管其确切的生物效应尚不清楚.

研究的目的:

  • 调查长时间暴露于vIL-6对染色体景观和细胞因子生产的生物学影响.
  • 阐明vIL-6在KSHV感染中导致慢性炎症的表观遗传机制.

主要方法:

  • 利用醇链化进行代谢测序和裂变在使用核酶 (TRAMS-CLART) 分析的目标和释放下.
  • 研究了长时间暴露于vIL-6对染色质可访问性,基因组修饰和转录因子结合的影响.
  • 评估了一种代蛋白和额外终端动机 (BET) 抑制剂 (OTX015) 对vIL-6诱导的细胞因子产生的影响.

主要成果:

  • 长时间的vIL-6暴露增加了含有4 (BRD4) 和乙化组合素H3素27 (H3K27ac) 的布罗马多在染色体上的同时占用.
  • 这些表观遗传变化与稳定的RNA聚合酶II (RNAPII) 的增加招募以及刺激后增强,持续的NF-κB p65结合有关.
  • 这导致hIL-6和IL-10的产量增加,由BRD4抑制剂OTX015.15逆转.

结论:

  • 持续暴露于vIL-6可能会诱导单细胞中的表观遗传记忆,建立有利于炎症反应的染色质状态.
  • 这种表观遗传修饰可以解释KSHV感染个体中观察到的慢性炎症疾病风险增加.
  • 准BRD4为缓解KICS相关炎症提供了一个潜在的治疗策略.