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在质细胞中BDNF和TrkB表达的遗传剖析

Changran Niu, Xinpei Yue, Juan Ji An

bioRxiv : the preprint server for biology

|July 28, 2023

概括

这项研究揭示了来自大脑的神经营养因子 (BDNF) 和其受体TrkB在微质体中没有表达. 星体细胞主要表达TrkB.T,而寡体细胞表现出多种表达模式,开辟了新的研究途径.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

大脑衍生神经营养因子 (BDNF) 和与热胺相关的激酶受体B (TrkB) 对神经元功能至关重要.
之前的研究报告了关于质细胞中BDNF和TrkB表达的相互矛盾的发现.
了解质细胞表达对于理解中枢神经系统功能和疾病至关重要.

研究的目的:

用先进的遗传方法精确地描述小鼠质细胞中BDNF和TrkB的表达.
为了澄清表达BDNF,TrkB.FL和TrkB.T.的特定质细胞类型.

主要方法:

使用了针对BDNF,TrkB.FL或所有TrkB异型的CRE特异性小鼠菌株,与CRE依赖EGFP记者小鼠交叉.
使用免疫组织化学与质细胞标记物 (微细胞,星球细胞,寡细胞) 来检测BDNF和TrkB的表达.

主要成果:

微质中没有检测到BDNF或TrkB的表达.
大多数成年星球细胞表达TrkB.T;一个子集表达BDNF.
基基细胞前体细胞表达TrkB.FL和TrkB.T,而成熟的基基细胞表达BDNF.

结论:

澄清了小鼠质细胞中BDNF和TrkB的精确表达模式.
在一组星球细胞和寡细胞中确定了BDNF的新表达.
开辟了研究BDNF和TrkB在质细胞生物学和中枢神经系统疾病中的特定作用的途径.

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