在蜂群中进行鞭毛运动改造需要FliL
Jonathan D Partridge1, Yann Dufour2, YuneSahng Hwang1
1Department of Molecular Biosciences and the LaMontagne Center for Infectious Diseases The University of Texas at Austin, Austin, Texas, 78712, USA.
bioRxiv : the preprint server for biology
|July 28, 2023
概括
FliL蛋白重塑细菌鞭毛电机,以增强像大肠杆菌这样的各种物种的群体运动性. 这种蛋白质对于增加细胞速度和抑制表面运动期间的翻转至关重要.
科学领域:
- 微生物学 微生物学
- 细菌的运动性 细菌的运动性
- 分子生物学分子生物学
背景情况:
- FliL是细菌鞭毛的组成部分,对其运动性至关重要.
- 它在蜂群中的作用,一种基于表面的运动,是有条件的,并且与运动功能有关.
- 蜂群需要增加鞭毛扭矩和生理适应.
研究的目的:
- 在蜂群期间,在各种细菌中识别FliL的共同功能.
- 调查FliL在细菌电机改造中对表面导航的作用.
- 探索FliL在不同细菌族群中的保存功能.
主要方法:
- 在大肠杆菌 (Escherichia coli),细菌 (Bacillus subtilis) 和神奇蛋白 (Proteus mirabilis) 中对FliL功能的比较分析.
- 在蜂群中评估细菌细胞速度和鞭毛运动偏差.
- 使用来自不同物种的fliL基因在E. coli突变体中的补充试验.
- 研究FliL与鞭毛MS环蛋白FliF的相互作用.
主要成果:
- 对于"运动重塑"来说,FliL是必需的,其特点是细胞速度增加和抑制在大肠杆菌,B. subtilis和P. mirabilis中蜂群期间的翻转.
- 蜂群和运动重塑表型在一个大肠杆菌突变体中恢复,并补充了来自P. mirabilis和B. subtilis的FLL基因.
- FliL和FliF之间的相互作用局限于FliF的RBM-3域,可能解释FliL相关的表型.
结论:
- FliL在重塑鞭毛电机方面发挥着保留作用,以在各种细菌物种中实现高效的蜂群运动.
- 与FliF的RBM-3域的相互作用对FliL在运动调节中的功能至关重要.
- 了解FliL的功能为细菌适应表面环境提供了洞察力.
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