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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Updated: Jul 21, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

一种微流体策略,捕获抗原特异性高亲和性B细胞.

Ahmed M Alhassan¹, Venktesh S Shirure¹, Jean Luo²

¹Department of Biomedical Engineering, University of California, Davis.

bioRxiv : the preprint server for biology

|July 28, 2023

概括

一种新的微流体方法快速评估B细胞抗原在强力下结合的亲和力. 这种方法可以识别用于免疫状况评估和临床应用的高亲和性B细胞.

关键词:

亲和力是一种亲和力.这就是贪,贪.细胞分离细胞分离免疫学免疫学免疫学

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Last Updated: Jul 21, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

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科学领域:

免疫学免疫学免疫学
生物物理学的生物物理.
微流体学微流体学

背景情况:

目前用于评估B细胞反应和抗体亲和力的方法通常是昂贵的,耗时的,并且不考虑力对结合相互作用的影响.
最近的研究表明,机械力可以调节B细胞受体 (BCR) -抗原结合,影响免疫状态评估.

研究的目的:

开发和验证一种微流体策略,以评估在生理相关力量下抗原特异性B细胞受体 (BCR) 的结合亲和力.
为了证明这种方法在丰富高亲和度B细胞和关联BCR-抗原相互作用与抗体溶液亲和度方面的实用性.

主要方法:

一个层状流动的微流体室是用固定的流感A型血凝素抗原设计的.
分析了五种血凝素特异性杂交瘤,以检测抗原特异性BCR结合亲缘关系,其强度范围为65至650 pN.
该方法评估了在不同的切削力下对BCR的结合,并测量了结合寿命.

主要成果:

增加剪切力和更长的结合寿命有效地丰富了抗原特异性,高亲和度B细胞.
在微流体室内测量的膜结合BCRs的亲和力与溶液中的相应抗体的亲和力有很强的相关性.
微流体策略证明了BCR抗原结合性质的快速评估.

结论:

微流体方法可以快速有效地评估BCR抗原结合亲和力,并识别高亲和力B细胞.
这项技术有可能从外围B细胞评估功能性免疫状态.
该方法提供了一种具有成本效益的方法,用于识别B细胞作为治疗抗体开发的来源.