Teosinte Pollen Drive指导着玉米的多样化和化通过RNAi
Benjamin Berube1, Evan Ernst1, Jonathan Cahn1
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor NY11724.
bioRxiv : the preprint server for biology
|July 28, 2023
概括
泰奥辛特花粉驱动,在玉米-泰奥辛特杂交的基因驱动,使用RNA干扰偏向通过花粉传输. 这种机制很可能影响了玉米的化和多样化.
科学领域:
- 遗传学 遗传学是一种遗传学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 介质驱动因素操纵生殖发育,以偏向自身的传播,挑战孟德尔遗传.
- 染色体驱动通常发生在雌性半体变异中,而基因驱动通常是后半体变异和男性特异性.
研究的目的:
- 描述Teosinte花粉驱动,一种在玉米-teosinte杂交中的新型基因驱动机制.
- 为了研究RNA干扰 (RNAi) 在这种基因驱动中的作用.
- 探索Teosinte Pollen Drive对玉米化和多样化的影响.
主要方法:
- 使用单分子和单花粉基因组测序.
- 研究了小RNA对Dicer-Like 2 (Dcl2) 的依赖性及其针对Teosinte驱动响应器1 (Tdr1) 的向性.
- 调查了玉米陆地种和神种群,以寻找与RNAi相关的基因中的混合模式.
主要成果:
- 鉴定了依赖于RNA干扰 (RNAi) 的Teosinte花粉驱动,在玉米-teosinte杂交物中.
- 证明了来自teosinte的非编码RNA发针中的22nt小RNA依赖Dicer-Like 2 (Dcl2) 并准Tdr1,这对于花粉活力至关重要.
- 发现了Dcl2,Tdr1和5号染色体上的头发针的密切伪链接,特别是在男性传播期间.
- 在玉米土地种和teosinte种群的多个染色体中观察到RNAi基因的相关混合模式.
结论:
- 泰奥辛特花粉驱动器可能在玉米的化和多样化中发挥了重要作用.
- 这种机制解释了植物和动物生殖系中"自我"小RNA的流行.
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