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细胞分裂角度调节组织机制,调整小脑折叠的数量

Amber G Cook¹, Taylor V Bishop¹, Hannah R Crowe¹

¹Mississippi State University, United States.

bioRxiv : the preprint server for biology

|July 28, 2023

概括

神经组织折叠,就像小脑一样,由差异扩张和层厚度来调节. 在外部颗粒层 (EGL) 中的细胞分裂角度调整这些因素以控制折叠量和波长.

科学领域:

发育生物学是发展生物学.
神经科学是一个神经科学.
生物物理学的生物物理.

背景情况:

神经组织折叠,特别是在小脑,是一个由机械力量影响的复杂过程.
以前的建模研究表明,组织层之间的差异扩张和外层厚度分别决定了折叠量和波长.

研究的目的:

研究神经组织折叠的拟议机制,使用具有不同小脑折叠的遗传特异性小鼠菌株.
确定关键的发育时期和调节大脑小脑折叠量和波长的细胞机制.

主要方法:

使用了表现出明显大脑小脑折叠模式的内生小鼠菌株.
在关键发育时期,分析了外部颗粒层 (EGL) 和小脑核之间的差异扩张的区域变化.
测量了EGL厚度,并将其与折叠波长相关联.
研究了SHH表达的Purkinje细胞和EGL增殖率.
检查了EGL内部的细胞分裂角度及其与触角扩张和EGL厚度的关系.

主要成果:

确定了一个关键时期,在此期间,小脑折叠量在小鼠菌株之间有所不同.
在EGL和小脑核之间的区域差异扩张与折叠量相关.
在此期间,EGL厚度在区域调整,与折叠波长的变化相关.
虽然SHH表达的Purkinje细胞数量预测了折叠数量,但EGL增殖率在各种菌株中是相似的.
在EGL细胞分裂角度的区域变化预测了接触式扩张和EGL厚度.

结论:

在EGL内部的细胞分裂角度作为一个可调的机制.
根据细胞分裂角度对差异扩张和EGL厚度的区域调整决定了小脑折叠量和波长.

关键词:

大脑的折叠脑的折叠细胞分裂角度的细胞分裂角度.小脑小脑是什么意思组织力学组织力学

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