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布鲁塞拉的细胞内生长是由Dps依赖的费里替诺法基激活的介导

Huynh Tan Hop¹, Tran Xuan Ngoc Huy², Hu Jang Lee²

¹University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.

|July 28, 2023

概括

布鲁塞拉细菌使用一种称为Dps的分泌的DNA结合蛋白来操纵宿主铁. 这种蛋白质通过增加铁的可用性和诱导细胞死亡来促进巨细胞内的细菌生存.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

布鲁塞拉物种是致病性细菌,会引起一种重要的动物传染病 - - 布鲁塞洛症.
布鲁塞拉病毒性依赖于逃避巨细胞内的宿主免疫反应.

研究的目的:

为了研究布鲁塞拉的DNA结合蛋白Dps在细胞内生存中的作用.
阐明Dps在宿主细胞内对布鲁塞拉病毒性有所贡献的机制.

主要方法:

研究了Brucella Dps在受感染的巨细胞中的定位.
评估了Dps对宿主铁平衡和反应性氧物种 (ROS) 生产的影响.
分析了ferritinophagy和Ncoa4基因在Brucella细胞内生长中的作用.
研究了Dps对宿主细胞死亡的作用.

主要成果:

布鲁塞拉Dps被分泌到巨细胞质中,调节宿主铁.
细胞质Dps抑制ROS的产生,并激活ferritinophagy,增加细胞内铁.
铁的增加促进了布鲁塞拉细胞的生长和宿主细胞死亡.
Ncoa4基因淘汰显著损害布鲁塞拉细胞内生长和宿主细胞死亡.

结论:

布鲁塞拉Dps通过操纵宿主铁代谢在毒性方面发挥了非常规的作用.
Dps通过ferritinophagy激活促进布鲁塞拉在巨细胞内的生存和繁殖.
准Dps-ferritinophagy轴代表了对抗牛病的潜在策略.

关键词:

布鲁塞拉病毒是什么?类似于费里的蛋白质 Dps.费里提诺法基 (ferritinophagy) 是一种对铁素的消化.铁的平衡是铁的平衡.巨细胞是一个巨细胞.

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