香白菌的内酶1与人类的CD4+T细胞结合,并调节原始和记忆反应
Muhammad Daud1, Prasad Dasari2, Marion Adelfinger3
1Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
European journal of immunology
|July 28, 2023
概括
坎迪达阿尔比坎斯 (Candida albicans) 酶1 (CaEno1) 与人类CD4+T细胞结合,促进真菌免疫逃避. 这种相互作用会损害对控制真菌感染至关重要的T细胞反应.
科学领域:
- 免疫学 免疫学 免疫学
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 由Candida albicans引起的危及生命的真菌感染对健康构成重大风险.
- 了解宿主-病原体相互作用对于开发有效的抗真菌疗法至关重要.
- 在C. albicans中,月光蛋白酶1 (Eno1) 与病毒性有关.
研究的目的:
- 为了研究Candida albicans enolase 1 (CaEno1) 和人类CD4+ T细胞之间的相互作用.
- 阐明这种相互作用对T细胞反应的功能后果.
- 探索CaEno1在真菌免疫逃避中的作用.
主要方法:
- 在子查真菌和宿主蛋白相互作用伙伴.
- 使用抗CD4单克隆抗体进行结合测定,以确定结合部位.
- 分析T细胞激活和细胞因子分泌概况 (Th2歪曲).
- 在记忆CD4+T细胞中对T细胞召回反应的评估.
- 针对CaEno1.1,对T细胞受体工程T细胞的体外评估.
主要成果:
- 生物信息预测证实了人类CD4细胞外域与C. albicans Eno1 (CaEno1) 的结合.
- CaEno1与CD4的细胞外域3结合,激活淋巴细胞特异性蛋白氨酸激酶 (LCK).
- CaEno1结合倾斜的天真CD4+T细胞向Th2细胞因子配置,表明真菌控制较差.
- CaEno1 抑制了人类的 CD4+ T 细胞回忆反应.
- 使用T细胞受体工程T细胞的采用T细胞疗法对CaEno1抑制产生了抵抗力.
结论:
- CaEno1直接调节CD4+T细胞的反应,有利于真菌的生存.
- CaEno1通过干扰补体调节器和直接调节T细胞免疫力来促进免疫逃避.
- 准CaEno1-CD4相互作用可能提供一种治疗策略来对抗Candida albicans感染.
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