相关实验视频

Updated: Jul 21, 2025

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Published on: November 17, 2023

在酵母细胞in situ中,异质的非正规核体占主导地位

Zhi Yang Tan¹, Shujun Cai¹, Alex J Noble²

¹Department of Biological Sciences and Center for BioImaging Sciences, National University of Singapore, Singapore, Singapore.

|July 28, 2023

概括

发芽酵母中的大多数核细胞并不在正规结构中. 细胞环境有利于非正规的核细胞体构造,DNA部分脱离.

科学领域:

细胞生物学细胞生物学
结构生物学结构生物学
生物化学生化学

背景情况:

核过程依赖于染色质组织,核细胞作为基本单元.
在体外 (in vitro) 中,正规的核细胞体结构定义得很好,但在体内 (in situ) 的结构仍然不太清楚.
哺乳动物核细胞的子集类似于正规结构,但这并不普遍适用.

研究的目的:

为了研究在发芽的酵母中核细胞的局部结构.
为了确定细胞环境中的正规核体的流行程度.
了解核内环境对核细胞构成的影响.

主要方法:

低温电子断层扫描 (cryo-ET) 用于可视化完整的芽酵母细胞内的核体.
使用3D分类分析对核细胞结构进行分类和分析.
实验是使用H2A-GFP作为唯一的 histone H2A来源的菌株进行的.

主要成果:

规范核体构成的核体在位总量的不到10%.
类似于正规核体的结构被观察到ex vivo,但不是in situ.
发芽酵母的内核环境有利于多个非正规的核细胞体构造.
有证据表明,DNA从平均芽酵母核细胞中部分脱离.

结论:

准则核细胞模型不足以描述芽起的酵母中的大部分in situ核细胞.
细胞环境显著影响核体结构,导致非正规的形式.
一种在平均发芽酵母核细胞中提出部分脱离DNA的模型在现场得到了研究结果的支持.

关键词:

它们中的一种是S. cerevisiae.细胞生物学细胞生物学在这种情况下,染色染色素染色体是一种染色体.化-ETET可以使用.结冷-FIB 的情况.基因表达的基因表达方式核子组中的核子

更多相关视频

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Published on: January 26, 2017

相关概念视频

Heterochromatin

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...

Euchromatin

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

Histone Variants at the Centromere

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.