一个低成本的基于微流体的检测设备,用于基于智能手机的生物标记物的快速识别和定量
Chonghui Yang1, Yujing Yang1, Gaozhen Zhao1
1State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Biosensors
|July 28, 2023
概括
我们创建了一个低成本的智能手机设备,用于快速,敏感的生物标志物检测,使用微流体. 这种快速诊断工具需要最小的样本量,并提供了早期疾病诊断的潜力.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断 临床诊断
背景情况:
- 敏感和快速检测微样本对于早期疾病诊断至关重要.
- 微流体芯片具有潜力,但面临着复杂的准备和高成本等挑战.
研究的目的:
- 开发一种基于智能手机的低成本光检测装置 (智能手机-BFDD),用于快速生物标志物分析.
- 克服现有的微流体诊断工具的局限性,专注于降低成本和复杂性.
主要方法:
- 在智能手机平台上集成微流体技术与RGB图像分析.
- 使用SARS-CoV-2 N-蛋白作为模型,在玻璃毛细血管内开发三明治免疫试验.
- 使用了较低的样品体积 (20μL) 和快速检测时间 (30分钟).
主要成果:
- 达到了SARS-CoV-2 N-蛋白的40 ng mL-1的低检测极限 (LOD).
- 使用RGB图像分析证明有效识别和定量生物标志物.
- 智能手机BFDD不需要精密设备,从而降低了整体成本和复杂性.
结论:
- 开发的智能手机-BFDD为生物标志物检测提供了具有成本效益和快速的解决方案.
- 这项技术对于生物医学研究和诊断中的各种生物标志物具有广泛的适用性.
- 在护理场所和早期疾病诊断中广泛使用的潜力.
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