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洞察lncRNA-mRNA联合表达概况和ceRNA网络在脂聚糖诱导的急性肺损伤中的情况

Yue Shen¹, Linjing Gong², Fan Xu¹

¹Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Current issues in molecular biology

|July 28, 2023

概括

这项研究通过分析基因表达,揭示了参与急性肺损伤 (ALI) 的新型长非编码RNA (lncRNAs). 这些lncRNA与炎症过程有关,为ALI提供了潜在的治疗点.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

长非编码RNAs (lncRNAs) 在急性肺损伤 (ALI) 中发挥作用.
在ALI中 lncRNAs 的精确生物功能和分子机制仍然不完全理解.
研究 lncRNA 表达特征可以阐明它们在 ALI 发病过程中的参与.

研究的目的:

在脂聚糖 (LPS) 刺激的人类肺上皮细胞 (BEAS-2B) 中对lncRNA和mRNA表达进行全球分析.
确定ALI中关键的lncRNA及其相关分子机制.
构建调节网络 (lncRNA-mRNA,ceRNA) 并确定潜在的治疗点.

主要方法:

高通量测序以确定全球lncRNA和mRNA表达特征.
生物信息分析包括lncRNA-mRNA联合表达网络,基因本体学,基因和基因组的京都百科全书,基因组变异分析和蛋白质-蛋白质相互作用网络分析.
竞争的内源RNA (ceRNA) 网络构建和实时定量反转录聚合酶链反应 (RT-qPCR) 验证.

主要成果:

在对照细胞和LPS刺激细胞之间,有433个lncRNA和183个mRNA的分别表达.
关键的 lncRNA 向在与炎症相关的生物过程中丰富的mRNA.
一个ceRNA网络确定了5个lncRNA,13个microRNA和12个mRNA;IL-1β,IL-6和CXCL8作为枢纽基因出现.

结论:

这项研究确定了在肺上皮细胞中对LPS刺激的反应中差异表达的lncRNA和mRNA.
这些发现突显了特定的lncRNAs在ALI发病过程中的重要作用,主要是通过炎症途径.
构建的调节网络提供了对lncRNA介导的分子机制的见解,并建议ALI的潜在治疗点.

关键词:

肺部急性肺损伤是什么 ceRNA 监管网络的监管网络.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式高通量RNA测序的高通量RNA测序炎症反应是一种炎症反应.长长的非编码RNAs.

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