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在使用氨酸血管时,在Liaison XL分析仪上的25-OH-维生素D免疫试验中未披露的干扰

Julie Jacobs¹, Frank Vanneste¹, Julie Hotton¹

¹Department of Laboratory Medicine, Europe Hospitals, Brussels, Belgium.

Scandinavian journal of clinical and laboratory investigation

|July 28, 2023

概括

样本类型显著影响维生素D测量. 肝素血在联络XL分析仪上显示出大量的阳性偏差,影响了患者的分类. 实验室专业人员必须意识到这种干扰,以便进行准确的25-OH-维生素D测试.

科学领域:

临床化学临床化学
实验室医学实验室医学
内分泌学在内分泌学.

背景情况:

准确测量25-OH-维生素D对于诊断和管理维生素D状态至关重要.
优化样品体积使用导致采用肝素血作为替代样品类型.
观察到的不一致的结果促使对维生素D试验的样本类型影响进行了评估.

研究的目的:

调查样品类型 (血清与肝素等离子体) 对25-OH-维生素D测量的影响.
为了比较使用不同样本矩阵的Liaison XL (Diasorin) 和Cobas e801 (Roche) 分析器的性能.
为了确定与肝素血使用相关的潜在干扰和偏差.

主要方法:

收集了34个配对的血清凝和肝素等离子体样本.
使用Cobas e801和Liaison XL分析仪测量了25-OH-维生素D水平.
进行统计分析,包括曼-惠特尼测试和偏差计算.

主要成果:

与血清相比,Liaison XL分析仪显示肝素血的显著正偏差 (57.5%) (p < .001).
较高比例的患者被归类为"正常"使用肝素等离子体在联络XL (79%对64%).
在将血肝素与血清进行比较时,Cobas e801分析仪显示出最小的偏差 (-0.2%).

结论:

氨酸血显著干扰了连接XL分析仪上的25-OH-维生素D测量.
实验室专业人员必须意识到这种干扰,以确保准确的维生素D测试.
需要进一步的研究来阐明这种观察到的干扰背后的机制.

关键词:

25-OH-维生素D是25-OH-维生素D的重要组成部分.25 基维生素 D 3 3 维生素 D 3 的含量.干扰干扰干扰肝素是一种肝素.氨酸在血中的作用免疫测试是一种免疫测试.实验室诊断诊断实验室诊断

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