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在下层白中,子遗传和性卵的胚胎发育

Xin Peng¹, Zahid Khan^1,2, Xiao-Min Liu¹

¹College of Life Sciences, Northwest University, Xi'an 710069, China.

|July 28, 2023

概括

这项研究揭示了甲虫胚胎发育中的关键差异,即甲虫物种的性卵和甲虫卵之间的关键差异. 基因表达显著影响卵细胞发育和分裂率,影响这些社会昆虫的繁殖成功.

科学领域:

昆虫学和社会昆虫生物学昆虫学和社会昆虫生物学
发展生物学发展生物学
遗传学和基因组学遗传学和基因组学

背景情况:

是一种高度社交的昆虫,具有复杂的繁殖策略.
对于昆虫的繁殖生物学来说,了解性卵和伴生卵的胚胎发育至关重要.
之前的研究还没有完全详细介绍 Reticulitermes aculabialis 和 R. flaviceps 等关键白物种的发育阶段和遗传基础.

研究的目的:

描述和比较Reticulitermes aculabialis和R. flaviceps.中的伴生和性卵的胚胎发育阶段.
研究基因表达在卵细胞发育和分裂率中的作用.
建立一个全面的数据库,对白的亲遗传和性卵子发育.

主要方法:

在白蛋中的胚胎发育阶段的观察和描述.
对形态差异的统计分析,包括微型数量.
单细胞转录组测序以分析从卵到幼虫阶段的基因表达 (pka, map2k1, mapk1/3,hgk, mkp, pax6).
使用KEGG,COG和GO数据库进行基因分析.

主要成果:

在R. flaviceps和R. aculabialis之间,卵子发育阶段的时间有显著的差异.
在R. flaviceps和R. aculabialis FF殖民地类型之间微型数量的统计学上显著的差异.
86%的 R. flaviceps 伴生卵在胚皮阶段停止发育.
在R. aculabialis FF中,关键基因 (pka, map2k1, mapk1/3,hgk, mkp, pax6) 的表达水平明显高于R. flaviceps FF.
与R. aculabialis FF.相比,R. flaviceps FF殖民地的卵细胞分裂率较低,成熟卵细胞也较少.

结论:

长卵细胞和胚胎发育受到特定基因表达的显著影响.
在R. flaviceps的伴生卵中的发育停滞突出了特定物种的生殖限制.
这项研究提供了R. flaviceps和R. aculabialis中的第一个伴生和性卵子发育数据库,为它们的生殖生物学提供了洞察力.

关键词:

网球术语的意思是指胚胎发育过程中的胚胎发育.基因表达的基因表达方式双胞胎遗传卵子双胞胎遗传卵子单细胞转录基因组测序

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