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葡萄糖-6-酸盐1-聚酶CrGlu6有助于Clonostachys chloroleuca的发展和生物控制效率

Binna Lv¹, Yan Guo¹, Xue Zhao¹

¹Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Journal of fungi (Basel, Switzerland)

|July 28, 2023

概括

葡萄糖-6-酸盐1-表皮酶 (CrGlu6) 与菌类寄生虫Clonostachys chloroleuca中的一个关键信号蛋白 (Crmapk) 相互作用. CrGlu6对于真菌生长,发育和植物病原体的有效生物控制至关重要.

科学领域:

菌类学菌类学是指菌类学.
植物病理学植物病理学
生物控制剂生物控制剂

背景情况:

Clonostachys chloroleuca是一种重要的真菌寄生虫,用于控制植物真菌病.
甲基因激活蛋白激酶 (MAPK) 途径调节真菌发育和应激反应,这对真菌寄生虫至关重要.
克洛洛卢卡菌中的Crmapk基因与真菌寄生虫有关,但其调节网络尚未完全理解.

研究的目的:

为了研究葡萄糖-6-酸盐1-表皮酶 (CrGlu6) 在C. chloroleuca.的真菌寄生虫和生物控制功能中的作用.
为了阐明CrGlu6与MAPK途径组件Crmapk之间的相互作用.

主要方法:

蛋白质与蛋白质相互作用测试以确定Crmapk.的结合伙伴.
在宿主殖民期间对CrGlu6的基因表达分析.
基因删除和补充实验以评估CrGlu6的功能.
评估野生型和突变菌株的抗真菌活性,真菌寄生虫能力和生物控制有效性.

主要成果:

CrGlu6被确定为一种与Crmapk相互作用的蛋白质,其基因表达在Sclerotinia sclerotiorum的殖民期间被上调.
缺乏CrGlu6导致异常的真菌形态,减少结合,损害菌寄生虫活动,降低生物控制效率.
补充恢复了正常的表型和生物控制能力.

结论:

CrGlu6在C.chloroleuca的生长,发育和真菌寄生虫疗效方面发挥着至关重要的作用.
这项研究揭示了代谢酶 (CrGlu6) 与真菌生物控制中的信号通路 (MAPK) 之间的新奇相互作用.
这些发现有助于更好地了解C.chloroleuca生物控制潜力的基础上的监管机制.

关键词:

克隆斯塔基斯 (Clonostachys chloroleuca) 是一种有毒的植物.这就是MAPKK的意思.斯克莱罗提尼亚斯克莱罗提尼亚斯克莱罗提尼亚斯克莱罗提尼亚生物控制生物控制葡萄糖-6-酸盐1-表皮酶菌类寄生虫的存在菌类寄生虫

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