原体及其他方面:从各种组织来源获得的人体ECM属性的全面比较,用于再生医学应用
Nashaita Y Patrawalla1, Nilabh S Kajave2, Mohammad Z Albanna2,3
1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA.
Journal of functional biomaterials
|July 28, 2023
概括
来自不同人体组织的细胞外基质 (ECM) 水凝具有独特的特性. 出生ECM水凝显示高细胞活性,而皮肤ECM水凝对于生物打印是最佳的,这突显了组织来源选择对再生医学的重要性.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 细胞外基质 (ECM) 是一种复杂的蛋白质和GAG网络,对组织结构和功能至关重要.
- 由于其固有的生物活性,ECM衍生生物材料为组织再生提供了有前途的平台.
- 了解ECM的源特异性对于开发有效的组织结构至关重要.
研究的目的:
- 为了比较来自人体皮肤,骨,脂肪和出生组织的ECM水凝的物理化学和生物特性.
- 评估这些ECM水凝对于细胞封装和生物打印应用的适用性.
- 为了确定组织来源对ECM水凝特性的影响,用于再生医学.
主要方法:
- 人体组织的脱细胞化以获得ECM.
- 关于ECM水凝的特征,包括氧的含量,SDS-PAGE,凝动力学和压缩模量.
- 评估带组织介质干细胞 (CMSC) 反应,包括代谢活动.
- 基于ECM衍生油墨的风病学分析和基于挤压的生物打印.
主要成果:
- 所有的ECM都实现了>90%的脱细胞化效率.
- 出生ECM的原含量与对照组相比较;皮肤ECM的压缩模量最高.
- 出生ECM中的CMSC显示出最高的代谢活性;皮肤ECM油墨最适合生物打印.
- 与原蛋白对照组相比,ECM水凝表现出较慢的凝动力学和剪切稀释特性.
结论:
- 根据组织来源,ECM水凝的物理化学和生物特性有很大差异.
- 出生ECM促进高细胞代谢活动,而皮肤ECM是生物打印的理想选择.
- 精心选择ECM组织源对于设计再生医学中的仿生组织结构至关重要.
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