阴离子流体相脂质体的蛋白冠状体损害了它们的完整性,而不是被细胞吸收
Daria Tretiakova1, Maria Kobanenko1, Anna Alekseeva1
1Laboratory of Lipid Chemistry, Department of Chemical Biology of Glycans and Lipids, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
脂质体药物载体形成蛋白质冠状体,影响稳定性,而不是细胞吸收. 阴性脂质和胆固醇改变蛋白质结合,影响体内脂质细胞的性能.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物运输 药物运输 药物运输
背景情况:
- 冠状蛋白影响脂质体的药物载体相互作用.
- 对不同脂质表面上的蛋白质沉积模式的理解有限.
研究的目的:
- 研究不同组成的脂质体上的蛋白质冠状形成 (蛋酸胆,POPG,胆固醇).
- 确定冠状元组合对脂质体稳定性和细胞吸收的影响.
主要方法:
- 通过大小排除色谱分离的脂质体-蛋白质复合体.
- 使用无标签LC-MS/MS进行蛋白质分析.
- 通过素释放试验评估的脂质细胞稳定性.
- 细胞吸收在内皮细胞和细胞中进行评估.
主要成果:
- 阴性脂质 (POPG) 和胆固醇含量改变了冠状病毒中的相对蛋白质丰度.
- 阴性脂质体 (40% POPG) 显示冠状体富含阴性蛋白质,稳定性降低.
- 胆固醇增强了血中的脂质体稳定性.
- 冠状病毒的组成差异极小地影响了细胞内皮细胞和细胞的细胞吸收.
结论:
- 蛋白冠状病毒主要通过损害粒子稳定性来影响脂质体药物载体的性能.
- 细胞吸收受到蛋白质冠状结构变化的影响较小.
- 研究结果强调稳定性是基于脂质体的药物递送性能的一个关键因素.
更多相关视频
12:15Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
00:11Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
相关概念视频
Membrane Fluidity
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH
A drug's pKa and the pH of the gastrointestinal (GI) tract play crucial roles...
Asymmetric Lipid Bilayer
Colloids
Membrane Domains
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
