循环外体miRNAs作为急性大动脉剖析的新生物标志物:一个诊断准确性研究
Dan Zhang1, Xiang Zhao, Baozhu Wang
1The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Medicine
|July 28, 2023
概括
循环外体微RNAs (miRNAs) 显示为急性大动脉剖析 (AAD) 的诊断生物标志物具有前途. 特定的miRNAs,特别是miR-499a-5p,提供高精度,组合显著提高诊断性能.
科学领域:
- 心血管研究研究心血管研究
- 分子诊断学 分子诊断学
- 生物标志物发现发现
背景情况:
- 急性大动脉解剖 (AAD) 是一个关键的心血管紧急情况.
- 需要新的诊断生物标志物,以便早期检测并改善患者的治疗结果.
- 外体微RNAs (miRNAs) 被研究为潜在的非侵入性生物标志物.
研究的目的:
- 在患有AAD的患者中识别差异表达的外体miRNA.
- 为了评估这些miRNAs的诊断效果,单独和组合.
- 探索miRNA目标在AAD病变发生过程中的功能性作用.
主要方法:
- miRNA微阵列测序以识别差异表达的外体miRNAs.
- 在AAD患者和对照者的血清中分析了8种特定的miRNA和4种蛋白质 (包括D-Dimer和矩阵金属蛋白-9).
- 接收器操作特征 (ROC) 曲线分析以评估诊断准确性 (曲线下的面积 - AUC).
- 生物信息预测miRNA目标和途径分析.
主要成果:
- 发现八种外体miRNA在AAD患者中被差异表达 (P < .05).
- miR-499a-5p,miR-202-3p和D-Dimer显示出高的诊断效率 (AUC>0.90),其中miR-499a-5p达到95%的准确性 (AUC = 0.99).
- 对miR-499a-5p和miR-202-3p的综合分析产生了98%的准确性 (AUC = 0.998),以及对矩阵金属蛋白-9的98%的准确性 (AUC = 0.996).
- 这些miRNAs的预测目标与AAD的病理生理学有关.
结论:
- 血清外体miR-499a-5p,miR-143-3p和miR-202-3p是AAD的潜在诊断生物标志物.
- 多种生物标志物的组合显著提高了对大动脉剖析的诊断灵敏度和特异性.
- 外体miRNAs为非侵入性ADD诊断提供了一个有前途的途径.
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