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在台湾南部的免疫受损患者中分离出抗环病毒的HSV-1:低患病率和新突变

Jhao-You Kuo¹, Chun-Sheng Yeh², Shih-Min Wang^3,4

¹Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Journal of medical virology

|July 28, 2023

概括

抗乙克洛维尔的简单疹病毒1型 (HSV-1) 在台湾的免疫受损患者中很少见. 这项研究确定了与阿西克洛维尔耐药性相关的UL23基因中的新突变.

科学领域:

病毒学病毒学
分子生物学分子生物学
临床微生物学临床微生物学

背景情况:

简单疹病毒1型 (HSV-1) 建立了潜伏期,并且可以复发,特别是在免疫功能低下的个体中,造成严重的死亡风险.
乙克洛维尔 (ACV) 是一种主要治疗方法,但可能出现耐药性,使患者管理复杂化.

研究的目的:

在台湾南部调查抗阿西克洛维尔抗性HSV-1的流行情况.
在HSV-1分离物中识别与阿西克洛维尔耐药性相关的遗传突变.

主要方法:

使用斑块减少试验 (PRA) 和DNA减少试验 (DRA) 来检测ACV耐药性的表型查.
抗性菌株中UL23和UL30基因的桑格测序.
对已识别的突变进行3D蛋白质结构预测.

主要成果:

观察到ACV抗性HSV-1的发病率较低:免疫功能低下的患者中为1.38%,整体为0.9%.
在一个免疫功能低下的患者身上发现了UL23基因 (乙胺基酶) 中的一种新型Y248D突变.
在免疫能力强的患者中发现了UL30基因 (DNA聚合酶) 中的三种未报告的突变 (683-688删除,R227H,A351D).

结论:

在台湾南部的免疫受损患者中,抗阿西克洛维尔抗性HSV-1的患病率较低.
新型Y248D突变可能会影响病毒胆氨酸激酶的功能,从而导致ACV耐药性.
这些发现有助于针对HSV感染和耐药性的临床管理策略.

关键词:

在HSV-1病毒中,抗阿西克洛维尔的耐药性免疫功能低下的患者.变种变种变种变种变种

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