离子对图像,i2i,是一个用于连续电离技术的质谱成像数据分析平台
Johan Lillja1, Kyle D Duncan1,2, Ingela Lanekoff1
1Department of Chemistry - BMC, Uppsala University, Uppsala, 752 37, Sweden.
Analytical chemistry
|July 28, 2023
概括
一个新的MATLAB应用程序,离子到图像 (i2i),简化了从连续电离源的质谱成像 (MSI) 数据的处理. 该工具处理复杂的数据集,为研究人员提供更快,更灵活的离子图像生成.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 计算化学计算化学
背景情况:
- 质谱成像 (MSI) 提供高灵敏度,选择性的分子分布数据.
- 处理MSI数据,特别是来自具有自动增益控制 (AGC) 的连续电离源的数据,存在重大挑战.
- 现有的软件往往缺乏针对连续电离MSI数据的专门处理,因此需要新的解决方案.
研究的目的:
- 引入离子到图像 (i2i),一种基于MATLAB的新型应用程序,用于处理MSI数据.
- 为满足连续电离源,AGC和高分辨率数据集的特定数据处理需求.
- 提供灵活有效的工具,用于从复杂的MSI数据中生成,处理和导出离子图像.
主要方法:
- 开发一个基于 MATLAB 的应用程序,名为 ion-to-image (i2i).
- 实施定量,有针对性和非有针对性的数据处理策略.
- 支持复杂的扫描功能,包括MSn和SIM数据,以及完整的扫描.
主要成果:
- i2i能够快速处理大型和复杂的MSI数据集,将处理时间缩短到几分钟.
- 该应用程序可以从MSI数据中生成离子图像,这些数据是使用连续电离源和AGC获得的.
- 为各种MSI应用程序提供数据处理和出口选项的灵活性.
结论:
- i2i为处理专门的MSI数据提供了有价值,灵活和高效的解决方案.
- 开发的软件解决了MSI数据处理工具中的一个关键缺口.
- 预计i2i将成为MSI软件领域的重要资产.
相关概念视频
01:19Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
800
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
800
01:21Chemical Ionization (CI) Mass Spectrometry
801
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
801
01:19MALDI-TOF Mass Spectrometry
4.9K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.9K
01:16Mass Spectrometers
5.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.7K
01:09Ion-Exchange Chromatography
585
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
585
01:20Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
519
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
519