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在泰国Maha Sarakham的Kosumpee森林公园从自由行长尾中分离出来的Staphylococcus aureus复合体的分子表征
Natapol Pumipuntu1,2,3, Tawatchai Tanee1,4, Penkhae Thamsenanupap1,4
1One Health Research Unit, Mahasarakham University, Maha Sarakham 44000, Thailand.
Tropical medicine and infectious disease
|July 28, 2023
概括
泰国的野生子携带多种 Staphylococcus aureus 菌株,包括 MRSA 和 MSSA,具有显著的毒性基因. 这些发现凸显了子作为传播这些细菌在环境中的潜在储库.
科学领域:
- 微生物学 微生物学
- 兽医科学 兽医科学 兽医科学
- 公共卫生 公共卫生
背景情况:
- 黄金葡萄球菌复合体,包括耐甲西林黄金葡萄球菌 (MRSA),耐甲西林黄金葡萄球菌 (MSSA) 和阿根廷黄金葡萄球菌,是人类和动物中重要的细菌病原体.
- 关于野生动物携带的这些葡萄球菌菌株的分子特征的数据有限,特别是泰国的长尾.
研究的目的:
- 为了研究在自由活动的长尾中发现的MRSA,MSSA和S. argenteus菌株的分子特征.
- 为了识别泰国的这些葡萄球菌分离物中存在的毒性基因.
主要方法:
- 从泰国Kosumpee森林公园的长尾的口腔粘膜中获得细菌分离物.
- 聚合酶链反应 (PCR) 用于检测孤立菌株中的特定毒性基因 (pvl,tst,hla,hlb,clfA,spa,coa).
主要成果:
- 总共分析了21个分离物:14个MRSA,5个MSSA,2个S. argenteus.
- 毒性基因clfA,coa和spaIgG结合区域在所有分离物中普遍存在.
- 这些野生的葡萄球菌分离物表现出独特的分子特征,病毒性基因的流行率很高.
结论:
- 该研究确定了MRSA,MSSA和S. argenteus菌株,这些菌株在长尾中具有显著的毒性基因配置.
- 这些发现表明,研究区域的野生可能充当携带者,并有助于病毒性葡萄球菌细菌的传播.
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