相关实验视频
Updated: Jul 21, 2025

A Proximal Culture Method to Study Paracrine Signaling Between Cells
Published on: August 28, 2018
癌细胞和尸细胞一样,通过分泌的微环境重新编程正常细胞
Shadi Rabiee1,2, Elham Hoveizi3,4, Mahmood Barati5
1Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
癌细胞释放酸性和有条件的介质,改变正常的纤维细胞行为. 结合这些介质挽救了细胞存活率,并通过表观遗传修饰减少了DNA低甲基化和亡.
科学领域:
- 细胞生物学 细胞生物学
- 癌症生物学 癌症生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 瘤微环境影响癌症的进展和转移.
- 癌细胞分泌的因素可以远程影响正常细胞.
研究的目的:
- 研究来自MDA-MB-231癌细胞的酸性和有条件介质对NIH/3T3正常纤维细胞的影响.
- 分析对细胞死亡机制和DNA甲基化的影响.
主要方法:
- 用酸性介质,条件介质或其组合对NIH/3T3纤维细胞进行治疗.
- 评估细胞存活率,代谢活动 (克雷布斯循环,线粒体活动),活性氧物种 (ROS) 水平.
- 分析细胞死亡途径 (亡,自) 和全球DNA甲基化.
主要成果:
- 添加酸性介质在条件介质中挽救了细胞存活率.
- 仅酸性介质就改变了远离克雷布斯周期的代谢活动,并没有增强线粒体活动.
- 与单独条件介质相比,组合介质降低了ROS水平,并将细胞死亡转向了自.
- 酸性介质诱导了显著的DNA低甲基化;组合介质与单独的条件介质相比,显示了DNA低甲基化和亡的减少.
结论:
- 来自癌症的酸性和有条件介质可以远程激活正常细胞中的信号通路.
- 这些介质诱导代谢和表观遗传变化,影响细胞死亡和DNA甲基化.
- 酸性和有条件介质的组合显示出一种保护作用,通过表观遗传重编程减轻了一些仅由有条件介质引起的有害变化.
更多相关视频
07:44Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
Published on: November 28, 2019
09:18Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion
Published on: April 4, 2025
相关概念视频
The Tumor Microenvironment
Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Cancer Stem Cells and Tumor Maintenance
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Cancer
Somatic to iPS Cell Reprogramming