蛋白质溶解生物标志物的时间过程 应对阻力,高强度间隔和同时进行运动的反应
Joshua S Godwin1, Guilherme D Telles2, Felipe C Vechin2
1School of Kinesiology, Auburn University, Auburn, Alabama.
同时炼 (CE) 不会增加肌肉蛋白质分解标志物比单独抵抗炼 (RE) 或高强度间歇炼 (HIIE) 更多. 这项研究研究了对这些运动类型的单次发作的蛋白质分解反应.
科学领域:
- 运动生理学 运动生理学
- 肌肉生物学 肌肉生物学
- 分子生物学分子生物学
背景情况:
- 同时炼 (CE),结合阻力 (RE) 和高强度间歇炼 (HIIE),提供了综合训练的好处.
- 人们担心,与单独使用RE相比,CE可能会阻碍强度增大和强度增加.
- 肌肉蛋白在CE中的分解机制尚不清楚.
研究的目的:
- 研究RE,HIIE和CE单次发作对骨肌肉蛋白质分解标记物的急性影响.
- 分析乌比基蛋白酶和calpain系统的活性.
- 检查与肌肉蛋白分解相关的基因的表达.
主要方法:
- 九名未受过训练的男性接受了RE,HIIE和CE的比赛,每个比赛都进行了一次.
- 肌肉活检是在运动前,运动后立即,运动后4小时和8小时收集的.
- 分析了FBXO32,TRIM63/MuRF-1和FOXO3mRNA表达,以及20S蛋白酶和calpain活动.
主要成果:
- 运动后FBXO32mRNA表达增加,与HIIE相比,RE和CE的总值更高.
- 卡尔帕因-2mRNA显示了边际时间效应,但时间点之间没有显著差异.
- 没有观察到TRIM63/MuRF-1或FOXO3mRNA的显著变化,也没有观察到20S蛋白酶或calpain活动.
结论:
- 一次CE的发作不会使骨肌肉蛋白解的标志物超过RE或HIIE的单次发作.
- 这些发现表明,与其组分相比,CE并没有本质上诱导更大的肌肉蛋白质分解.
- 需要进一步的研究,以了解长期蛋白质分解适应CE培训.
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