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MYOD诱导的Inc-MEG3通过与DLST相互作用促进猪卫星细胞分化

Quan Liu^1,2, Mengxun Li¹, Su Xie¹

¹Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, P. R. China.

|July 28, 2023

概括

肌源性因子MYOD诱导长非编码RNAMEG3,然后增强二利胺糖转移酶 (DLST) 蛋白表达,促进肌肉细胞分化和骨肌肉再生.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

长非编码RNAs (lncRNAs) 是细胞过程的关键调节者,包括肌肉细胞的分化.
已知长非编码RNA-MEG3 (lncRNA-MEG3) 促进猪骨肌卫星细胞 (PSC) 的分化.
通过MEG3影响PSC分化,特别是其蛋白相互作用的精确分子机制需要进一步阐明.

研究的目的:

调查猪骨肌肉卫星细胞分化中的lncRNA-MEG3的调节机制.
为了识别和描述lncRNA-MEG3.3的蛋白标.
为了阐明 lncRNA-MEG3.3 的上游转录调节.

主要方法:

通过RNA拉下和RIP-qPCR测定来确定MEG3蛋白相互作用.
淘汰和过度表达实验,以评估DLST在PSC分化中的功能作用.
染色体免疫沉 (CHIP) 和光酶记者测定验证了MYOD与MEG3促进体的结合.
在体内对MEG3进行淘汰,以评估其在骨肌肉再生中的作用.

主要成果:

MEG3直接与二利胺糖转移酶 (DLST) 结合.
DLST促进PSC分化,其蛋白质表达受MEG3.3的调节.
肌原分化 (MYOD) 作为转录因子,与MEG3促进体结合并增强其转录.
在体内,MEG3对于骨肌肉的再生至关重要.

结论:

MYOD通过转录上调调节MEG3的表达.
MEG3充当分子支架,结合DLST并促进其蛋白质表达,从而增强PSC分化.
这项研究揭示了一种涉及MYOD,MEG3和DLST的新型分子机制,用于调节骨肌肉分化和再生.

关键词:

在 DLST 里面,在MEG3中,MEG3是MEG3,MEG3是MEG3.这就是MYOD的意义.公共服务中心 (PSCs) 的服务.不同化的差异化差异化.

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