德费里--原 (IX):一种对抗Mycobacterium abscessus的有希望的治疗方式
Sholeh Feizi1, Muhammed Awad2, Roshan Nepal1
1Department of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, Australia; The University of Adelaide, Adelaide, Australia.
一种新的组合疗法,deferiprone-gallium-protoporphyrin (Def-GaPP),显示出对Mycobacterium abscessus的协同活性,Mycobacterium abscessus是一种导致囊性纤维化患者肺部疾病的细菌. 这种Def-GaPP治疗在实验室研究中是安全和有效的,为新的非结核性菌根性肺病治疗提供了希望.
科学领域:
- 传染性疾病 传染性疾病
- 肺部病理学 肺部病理学
- 抗菌研究 抗菌研究
背景情况:
- 由Mycobacterium所引起的非结核性菌根性肺病 (NTM-PD) 是囊性纤维化 (CF) 患者的严重并发症.
- 目前对NTM-PD的治疗方法有限,迫切需要新的治疗策略.
- 原 (IX) (GaPP) 通过扰乱铁的吸收,已经显示出对抗多药耐药的M.腹的潜力,但其在非铁缺乏症条件下的有效性尚不清楚.
研究的目的:
- 在更具生理相关的环境中研究Def-GaPP对M.的协同抗菌活性.
- 评估Def-GaPP组合疗法的体外安全性和细胞毒性.
- 探索NTM-PD的新型治疗方法.
主要方法:
- 被M. abscessus感染的巨细胞被用deferiprone (Def) 和-protoporphyrin (IX) (GaPP) 组合治疗.
- 评估了细菌的活力,以确定协同作用.
- 在实验室中,在有效的抗菌度下,对Nuli-1和THP-1细胞系使用MTT测定来评估细胞毒性.
主要成果:
- Def-GaPP组合显示出对感染M.的巨细胞的协同活性.
- 用Def-GaPP治疗降低了高达0.9 log10的细菌活力,其度为10mM-12.5 mg/L.
- 在有效的抗菌度下,Def-GaPP对Nuli-1和THP-1细胞系没有显著的细胞毒性.
结论:
- Def-GaPP组合疗法显示出对M. abscessus有前途的协同抗菌作用.
- 该治疗在体外耐受性很好,这表明它具有良好的安全性.
- Def-GaPP需要进一步研究,作为一种潜在的新型抗菌剂,用于治疗非结核性菌根性肺病.
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