经EGF表达的人类介质干细胞抑制了角质细胞中的原酶1表达
Jeong Hyun Lee1, Gayathri Chellasamy2, Kyusik Yun2
1Department of Biological Sciences, Gachon University, Seongnam, South Korea.
Cellular signalling
|July 28, 2023
概括
人类表皮生长因子转移的介质干细胞 (hEGF MSCs) 增强了角质细胞的增殖和伤口愈合. 这项研究揭示了hEGF MSCs通过MAPK途径减少矩阵金属蛋白酶-1 (MMP-1) 表达.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 皮肤病学 皮肤病学
背景情况:
- 已知介质干细胞 (MSCs) 通过膜信号传递来进行组织修复.
- 人类MSC显示了各种疾病的治疗潜力.
- 皮肤生长因子 (EGF) 和MSCs对皮肤细胞的协同作用尚未被探索.
研究的目的:
- 研究人类表皮生长因子转移的MSCs (hEGF MSCs) 对人类角质细胞 (HaCaT) 细胞增殖的影响.
- 阐明调节矩阵金属蛋白酶 (MMP) - 1表达在用hEGFMSCs治疗的HaCaT细胞中的机制.
- 探索皮肤疾病治疗和化品的潜在应用.
主要方法:
- MSCs与人类EGF (hEGF) 等离子体的感染,通过凝电泳和qPCR证实.
- 使用WST-1和伤口愈合试验评估HaCaT细胞的增殖和迁移.
- 组图,2-DE分析,以及西部涂抹来分析MMP-1表达,原酶活性,碳氧酶和MAPK信号通路.
主要成果:
- 与MSC-CM相比,hEGF的MSC受条件介质 (CM) 显著增加了HaCaT细胞的增殖和伤口愈合率.
- hEGF MSC-CM治疗降低了原酶活性和MMP-1 mRNA和蛋白质水平.
- 碳氧酶的表达升高,而MAPK通路蛋白 (pErk,pJNK,p-p38) 和转录因子 (pCREB,NFκB,p-c-Fos) 降低,pAkt.
结论:
- hEGF MSCs有效地促进人类角质细胞的增殖,并增强伤口愈合.
- 该机制涉及MMP-1表达的下调,可能是通过基因激活蛋白激酶 (MAPK) 途径.
- 这些发现支持hEGF MSCs作为治疗皮肤疾病和化品应用的治疗剂的发展.
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