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卡本达暴露抑制了小鼠卵子中介成熟 in vitro 通过破坏组件组件来抑制小鼠卵子中介成熟

Cong Ma¹, Zhuonan Yang¹, Shouxin Zhang²

¹Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei, 230032, China; Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Ministry of Education of the People's Republic of China, No.81 Meishan Road, Hefei, 230032, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|July 28, 2023

概括

碳丹胺杀菌剂通过阻碍极体挤出和引起介质性停止来破坏小鼠卵细胞成熟. 这种真菌杀菌剂暴露会影响线组装,染色体对齐和精子结合,降低卵细胞质量.

科学领域:

生殖毒理学生殖毒理学
细胞生物学细胞生物学
环境科学环境科学

背景情况:

卵细胞质量对于受精和胚胎发育至关重要.
一种常见的杀真菌剂 - - 碳丹胺 (CBZ) 存在环境风险.
了解CBZ的生殖毒性至关重要.

研究的目的:

为了研究Carbendazim (CBZ) 对小鼠卵细胞成熟的影响.
确定CBZ影响环境变化的具体机制.

主要方法:

暴露小鼠卵细胞的碳班达 (CBZ).
分析介质性进展,细胞周期分布和/染色体动态.
评估p-MAPK水平,动脉核-微管 (K-MT) 连接,以及螺旋组装检查点 (SAC) 激活.
评价微管稳定性,基因素修饰和雌激素受体α (ERα) 分布.

主要成果:

CBZ阻碍了极体挤出 (PBE) 并导致I (MI) 阶段的转化停止.
CBZ破坏了螺旋组装,染色体对齐和K-MT附着,降低了p-MAPK水平并激活了SAC.
CBZ增加了α-tubulin乙化,降低了微管稳定性,改变了ERα分布,并影响了基因组修饰.
尽管一些卵细胞成熟了,但它们显示出螺旋/染色体异常和精子结合能力降低.

结论:

碳丹胺 (CBZ) 暴露会破坏卵细胞中的关键介质过程.
通过影响线动态,染色体对齐和受精潜力,CBZ会影响卵细胞质量.
这些发现凸显了Carbendazim (CBZ) 的生殖毒性.

关键词:

这种碳化合物是不必要的.基质子的修改基质子的修改肌肉分裂 (Miosis) 是一种卵细胞是卵细胞中的一个.螺旋组装检查点螺旋组装检查点

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