德赛赛尔格雷林调节GHS-R1受体表达和胎盘BeWo细胞中的细胞分化
Vanessa Coria-Caballero1, Maria-de-la-Luz Jaramillo-Narvaez2, Ma Guadalupe Leon-Verdin3
1Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus, Mexico.
Molecular and cellular endocrinology
|July 28, 2023
概括
德赛赛尔格雷林 (DAG) 通过抑制分化和改变生长激素分泌受体1 (GHS-R1) 的表达,影响胎盘细胞功能. 这些发现表明DAG在胎盘和胎儿生理学中起作用.
科学领域:
- 内分泌学 在内分泌学.
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
背景情况:
- 格林及其受体 (GHS-R1) 涉及到胎盘和胎儿的生长.
- 脱格瑞林 (DAG) 的特定作用,一个未化格瑞林形式,在胎盘功能仍然不清楚.
- 研究DAG对胎盘细胞的影响对于理解胎儿发育至关重要.
研究的目的:
- 为了确定desacylghrelin (DAG) 对人类热囊细胞 (BeWo) 细胞活力,增殖和分化的影响.
- 分析DAG对胎盘细胞中生长激素分泌受体1 (GHS-R1) 表达的影响.
- 为了阐明DAG在BeWo细胞中对GHS-R1的作用机制.
主要方法:
- 贝沃细胞被3nMDAG治疗了12,24,48,72小时.
- 评估了细胞活力,增殖,分化 (通过hCG量化) 和GHS-R1表达.
- 使用一种特定的抗剂 ([D-Lys3]-GHRP-6) 调查了GHS-R1的作用.
主要成果:
- DAG没有影响BeWo细胞活力或增殖,但抑制了分化.
- DAG最初在12小时和24小时刺激了GHS-R1的表达,但在48小时后抑制了它.
- DAG降低了细胞内和分泌的hCG水平,而GHS-R1抗剂调节了这些效应.
结论:
- 脱格瑞林 (DAG) 影响胎盘细胞分化和GHS-R1表达,可能是通过涉及GHS-R1本身的反机制.
- DAG对分化的抑制作用表明DAG在调节胎盘和胎儿生理学方面可能发挥作用.
- 需要进一步的研究才能充分理解DAG对妊娠结果的贡献.
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