在头角状细胞癌中长非编码RNA的异常表达和相关的调节机制
1Department of Maxillofacial Surgery, Stomatological Hospital of Tianjin Medical University, Tianjin, China.
概括
长非编码RNA (lncRNA) LAMB1-210在头部和部状细胞癌 (HNSCC) 中过度表达. 下调LAMB1-210抑制HNSCC细胞的生存,迁移和入侵.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 头部和部状细胞癌 (HNSCC) 是一种具有复杂分子基础的流行癌症.
- 长非编码RNAs (lncRNAs) 正在成为各种癌症的关键调节者,包括HNSCC.
研究的目的:
- 研究特定的lncRNA,LAMB1-210在HNSCC的发展和进展中的作用和机制.
- 探索LAMB1-210作为HNSCC.的治疗点的潜力.
主要方法:
- 对HNSCC组织和相邻的正常组织进行RNA测序,以识别差异表达的lncRNA和microRNA (miRNA).
- 定量实时聚合酶连锁反应 (qRT-PCR) 用于验证lncRNA表达.
- 功能性测试,包括细胞活力 (MTT),迁移 (伤口愈合) 和入侵 (Transwell),在短毛RNA (shRNA) 介导下调LAMB1-210后在HNSCC细胞系 (SCC-4和CAL-27) 中进行.
主要成果:
- 发现大量的lncRNAs和miRNAs在HNSCC和正常组织之间表达不同.
- 与人类口腔角质细胞 (HOK) 细胞相比,lncRNA LAMB1-210在HNSCC细胞系 (FADU,SCC-4,CAL-27) 中显著上调.
- 使用shRNA降低LAMB1-210的调节导致SCC-4和CAL-27细胞细胞活力,迁移和入侵的显著抑制.
结论:
- 与正常组织相比,lncRNA和miRNA表达特征在HNSCC组织中发生变化.
- 在HNSCC瘤细胞中,LAMB1-210过度表达,在促进细胞存活,迁移和入侵方面发挥着至关重要的作用.
- LAMB1-210代表了HNSCC.的潜在治疗点.
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