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克鲁佩尔样系因子9的丧失使得生理基因表达和发育失调

Laura Drepanos¹, Ian M Gans^2,3, Janelle Grendler²

¹Colby College, Waterville, ME, USA.

Scientific reports

|July 28, 2023

概括

克鲁佩尔类因子9 (Klf9) 的丧失会根据每天的时间不同地影响斑马鱼的基因表达. 在Klf9表达的黎明前峰值时,观察到对肝脏和神经元发育的特定影响.

科学领域:

发育生物学发展生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

克鲁佩尔类因子9 (Klf9) 是一种调节压力和内分泌通路的转录因子.
之前的研究分析了Klf9在单一时间点 (受精后5天) 的转录基因效应,这与其最低表达水平相吻合.

研究的目的:

研究Klf9表达的日间振荡如何影响Klf9缺乏的转录组后果.
为了确定Klf9功能丧失对斑马鱼发育的日间特定影响.

主要方法:

在3个时间点对5天的受精后斑马鱼胚胎进行大量RNA测序,捕获Klf9表达峰值和最低点.
与单细胞RNA测序图谱集成,以映射特定细胞类型的转录组变化.
对焦成像评估 Klf9 突变斑马鱼的器官形态.

主要成果:

Klf9 缺乏对日间不同时间的基因表达产生强烈影响,但在黎明前的峰值观察到额外的影响.
Klf9突变与消化器官 (肝脏,胰腺,肠道) 的mRNA增加以及分化神经元和血液中的mRNA减少有关.
在Klf9突变幼虫中观察到肝脏膨胀,与肝脏mRNA水平增加相关.

结论:

Klf9缺乏的转录效应部分取决于白天的时间,在黎明前的表达峰值有独特的影响.
在斑马鱼中,Klf9在调节消化器官,神经元和血液细胞的发展方面发挥着至关重要的作用.
干扰Klf9的功能导致肝脏过度生长,突出其在肝脏发育和恒温的重要性.

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